The Therapeutical Effect Of Basic Fibroblast Growth Factor On The Rat Retinal Ischemia-Reperfusion Injury

Objective To investigate the action mechanism of basic Fibroblast Growth Factor(bFGF) injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury,and provide theoretical basis to the therapeutical use of bFGF on retinal ischemia/reperfusion injury. Method 48 rats were divided into normal, ischemia and treatment group randomly. The rat model of experimental retinal ischemia/reperfusion injury was made by increasing the intraocular pressure. Atomic absorption spectrum method was taken to evaluate the inner cellular calcium changes of retina tissues. The change of free radical of retina tissues was detected by enzyme chemistry method . The expression of caspase-3 of different time courses was determined by SP immunohistochemistry. Result l.The intracellular calcium level of rat retina began to increase at Olh hour after reperfusion, and went on increasing with the reperfusion time prolonged. At 24lh hour after reperfusion the intracel lular calcium level reached the peak, and began to decease at 72h hour, but it was still quite high. At 6th hour after reperfusion the difference of intracellular calcium between ischemia group and treatment group was of statistical significance. 2. The MDA and SOD levels changed at the early time courses after retina i schemia-rcpcrfusion injury. At Olhhour after reperfusion the MDA level increased siightly, while the SOD level decreased slightly. With the time going on, the MDA level increased gradually while the SOD level decreased gradually. The increase of MDA level and the decrease of SOD level were parallel, and the peak was reached at 24lh hour after reperfusion. At 72lh hour after reperfusion the MDA level had droppedback for some extent, while the SOD level had gone up a bit, and they were obvious different from normal retina tissue. After 6lh hour after reperfusion the difference of MDA level and SOD level between ischemia group and treatment group was of statistical significance. 3. At 6"1 hour after reperfusion ,caspase-3 gene began to express in a small quantity in the retina ganglion cell layer and inner nucleous layer. The expression reached the peak at 24lh hour after reperfusion, and at 72th hour there was no obvious caspase-3 expression. The change trend of treatment group was similar except that the expression amount was relatively obvious less. There were statistical significance (P < 0. 05) between the ischemia group and treatment group at 12'h and 24lh after reperfusion. Conclusion Retina ischemia-reperfusion injury can cause the increase of retina intracellular calcium and free radicals in the retina;the expression of caspase-3 protein increase after retina ischemia-reperfusion injury;bFGF can inhibit the increase of retina intracellular calciums and free radicals , and it can also reduce the increase of the expression of caspase-3 protein.Postgraduate Zhao Yansong(Ophthalmology) Directed by Prof. Niu Yingjun...