Experimental Studies Of Pharmacodynamics On Senqi Naobao's Therapy For Focal Cerebral Infarction In Rats

Adjective:To study the treatment effect of Senqi Naobao on focal cerebral ischemia in rats and the relationship between the curative effect and dose, observe its prevent effect on microvascular thrombosis in mesentery by using three different animal models. Furthermore we observed the roles of Senqi Naobao's nutrition and the anti-apoptosis on PC12 cells. We expected to identify the effect of Senqi Naobao on treatment of brain ischemia by animal experiment.Methods:1. By using the model of photochemistry-inducing focal cerebral ischemia in rats, we observed dynamical the change of weight and neurologic score started from 3 hours after the onset of ischemia. With Senqi Naobao pouring into stomach 2ml per day for 5 days, the rat brain was excised on day 5 after ischemia , , TTC staining was used for measuring infarct volumes and HE staining was used for observing the pathological change.2. By using the model of thread embolizing-inducing focal cerebral ischemia-reperfusion in rats, we observed the ratio of death within 48 hours, the ratio of survival in 7 days, the infarct volume and pahthological alteration 7 days after reperfusion with Senqi Naobao poured into stomach for 7 days.3. A model of microvascular thrombosis initiated by a photochemicalreaction in mesentery was used to observe the effect of Senqi Naobao on thrombosis in mesentery on sixth day with Senqi Naobao poured continually into stomach for 5 days before the experiment. 4. The roles of nutrition and anti-apoptosis of the rat serum containing Senqi Naobao on PC12 cell was also observed following the continual administration by using the methods of cell culture and H2O2-inducing cell apoptosis.Results:1. Senqi Naobao significantly reduced the volume of the infarct in large dose group and middle dose group compared with control (p<0. 001). The curative effect of middle dose group is more effective than Ginnaton group. The relationship between dose and effect demonstrated that large dose and middle dose is effective, however no significant differences were revealed between small dose group and control group(P>0. 05).2. At Senqi Naobao group , the ratio of death 48 hours after ischemia reperfusion was lower than control group, and the ratio of survival 7 days after reperfusion was higher than control group. The infarct volume was significantly reduced in Senqi Naobao group compared with control(p<0.001).3. The length of the thrombosis in mesentery microvascular illuminated by light in Senqi Naobao group is shorter than that of control within the time windows of 1 min and 10 min. At the same time point, the area ratio of thrombus to vein is also smallerthan control.4. The number of PC12 cells was all increased significantly in the three doses groups of Senqi Naobao compared with control (P<0. 001). They also can suppress neuronal apoptosis induced by H202. Among the three dose groups, the effect of large dose group and middle dose group are stronger than control.Conclusion:The results shown that Senqi Naobao has a significant beneficial effect on focal cerebral ischemia induced by photochemistry in rats. It also can decrease the brain's damage associated with cerebral ischemia-reperfusion. The rat serum containing Senqi Naobao has the preventive effect on microvascular thrombosis; furthermore it can stimulate the proliferation of PC12 cells, and suppress neuronal apoptosis induced by...