Morris Maze Performance, TPA Activity And Microtubule In The Vascular Dementia Rat

Purpose :To investigate factors on Morris maze memory performance, observe the change of TPA activity and microtubule in Vascular dementia rats. Methods: Two kind animals, different sex andold rats were practiced in Morris maze. Vascular dementia rats model were made by ligation bilateral common carotid arteries . TPA activity was measured by ELISA. (3 -tubulin antigen was detected by immunohistochemistry. Microtubule was observed by electron microscopy. Result: mouse is not suitable for Morris maze performance. Factors as sex, old and ligation affect the performance. TPA activity is high early in either ligation or control rats, but it is lower in lagition later. After ligation, numbers of the hippocampal CA1 neuron and (3 -tubulin immunohistochemical stain are decreased. MT change was also seen by electron microscopy. Conclusions: To establish ischemia animal mode for memory study, appropriate animal should be selected. Factors as sex and old should be under estimate. Ligation both common carotid arteries of rats is a way to make vascular dementia model. Abnormal TPA activity may involve in pathology of neuron. Microtubule is vulnerable and its structure integrity is important for neuron function. There may be relationship among TPA activity, microtubule and memory.