Animal Experimental Research On Tang-Zhi-Ping Treating Diabete

1. The effect of herba TZP on blood sugar in normal and experimental diabetic mice. The blood glucose level in mice was monitored after ig. TZP at doses of 3.5, 7.0 and 14.0g/kg, l/dX5. which indicates that TZP can keep the stability of empty-stomach blood glucose in normal mice and significantly decrease the blood glucose of diabetic mice caused by adrenalin,glucose and alloxan. Besides, the largest enduring dose of mice daily is 68.75g/kg after ig.TZP, which is 395 times more than adult The above explains that TZP contains little toxin.2.The effect of herba TZP on glucolipide metabolism and function of islets of pancreas in experimental NIDDM diabetes mellitus. SD rats were fed with a high quantity of heat diet enriched with peanut oil(10%,w/w), triglyceride (10%,w/w) and condensed milk (15%,w/w). After one month on the diet, STREPTOZOTOCIN(stz, 33mg/kg) was injected into tail-vein to develop experimental fat and diabetic rats. Meanwhile, the rat models were treated with TZP at dosed of 3.5, 14.0g/kg , l/dX36. Results show: TZP can clearly decrease the weight-growth rate and the level of serum triglyceride in the rat models, and reduce the empty-stomach blood glucose rising and inhibit blood glucose growing caused by glucose loading test Ih, 3h. Besides, TZP can cause hyperinsulin and hyperglycemic trend to decreasing [compared with negative control groups, the serum insulin and glucagon in the groups of higher dose of TZP decreased by 15.29% (PXX05) and 21.81% (PO.05) separately), which indicates TZP can inhibit secretion of serum glucagon and growth of weight, improve disturbance of glucolipidemetabolism so that it can lower insulin resistance in the rat models.'3.The effect of TZP on polyols in sciatic nerve and lens of diabetic rats. Alloxan 40mg/kg was injected into SD rat' tail vein to develop alloxan diabetic rat models, which were treated with TZP at doses 4.7 and 14.0g/kg, 1/d X 30. Results are that TZP can significantly decrease the content of blood glucose and glucose in sciatic nerve and lens of diabetic rat models. TZP is effective to reduce sorbitol content in sciatic nerve and lens(compared with negative control groups, the level of sorbitol content in the groups of higher dose of TZP decreased by 23.80% and 26.42% separately, 0.05