| The periodontal tissue restoration and regeneration is a problem that perplexes the periodontium scholars over a long period of time. In recent years, abroad scholars applied amelogenin in periodontal intrabony defects for reestablish periodontal tissue. It is said that the result was quite inspiring. However, the mechanism that amelogenin induces periodontal tissue regeneration is unclear. And in China, the study of amelogenin in periodontal tissue regeneration is even being in primary stage. The purpose of the present study was to explore amelogenin's cytobiology mechanism in periodontal tissue regeneration and to investigate the possibility of amelogenin in clinical use.We extracted enamel matrix proteins (EMPs) whose main component is amelogenin from porcine tooth germ, and identified it's biological activity, then observed some important effects of EMPs on the periodontal ligament cells through improved cell culture technique. After that, we applied EMPs in 11 volunteers with periodontitis for a primary clinical outcome of EMPs. The methods and results of every experiment is as followed:1.Extraction of EMPsDissected teeth germ from fresh jaws of porcine with age 6 mouths, and then we scraped them to obtain the unmineralized enamel matrix. After that, EMPs was extracted by acetic acid method. The proteins we got showed typical molecular weight distribution of amelogenin by using SDS-PAGE, that is to say, the molecular weight distribution collected in 20 1 |
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