| Objective To observe the changes of transforming growth factor beta 1 expression in the contralateral dorsal root ganglion(DRG)after a acute conditioning lesion and chronic injury in the rats and learning about the function of TGF- ?1 in peripheral nerve injury and repair,to observe the length of the contralateral crushed nerve regeneration and learning about the correlation on the conditioning lesion and nerve regeneration.Methods (l)Acute injury modal: Mate Sprague-Dawley rats were randomly divided into two groups. In group A, the left sciatic nerve was exposed but not crushed. In group B, it was crushed. After 5 days, the right sciatic nerves of all rats were crushed. The histology and immunohistochemistry examination were performed respectively after 0> 1 -, 3 5 7 days injury.(2)Model of chronically compressed peripheral nerve: Mate Sprague-Dawley rats were used.The right sciatic nerves of all rats were compressed .The histology and immunohistochemistry examination were performed respectively after K 2% 4> 6, 8 weeks injury.Results The expression of TGF- 1 slowly increased in both groups, and peaked at 5th day in the acute injury model. From then on, it decreased gradually. The initial delay of experimental group, 1.05 days, wassignificantly shorter than that of the control group, 1.70days(p<0.05). The expression of TGF- P 1 slowly increased in both groups, and peaked at week 4 in the model of chronically compressed peripheral nerve . From then on, it decreased gradually.Conclusions In the sciatic nerves of rats, a conditioning lesion promotes contralateral nerve regeneration by shortening the initial delay and enhances the expression of TGF- P 1 in the contralateral nerve cell body. The TGF- P 1 not only participate in the pathophysiological process of peripheral nerve damage, but have some effects on peripheral nerve repair as well.
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