| Objective: To observe the expression of nerve growth factor and to determine the correlation between diabetic cystopathy and the expression of nerve growth factor in the bladder and lumbosacral dorsal root ganglion by using diabetic model. Furthermore, the pathogenesy of DC and the role of NGF in the development of DC were explored in this study, which would establish the foundation for us to cure DC by using NGF.Methods: Thirty five adult female Sprague-Dawley (SD) were divided into two groups at random: rats weight 200-250g, the normal control group (n=15), the diabetic model group (n=20). The diabetic model rats were induced by injection of the streptozotocin (STZ), 60mg/kg. The normal control group was set up. The two groups rats were feed for common forage and were taken food and water freely. Diabetes was confirmed in STZ-injected rats by measurement of plasma glucose concentration in blood samples from the tail vein. The blood glucose (BG) of tail vein were measured after 72 hours of injection of STZ, when BG>16.7mmo1/L. The experiments were carried out eight weeks after the model, was successfully set up and the blood glucose of tail vein were measured again and those rats (BG≤16.7mmol/L) were removed. The twenty four'voiding volume of the rats in the metabolic cage was determined one day before the experiment. The bladders and DRGs from both sides of L6-S1 were completely cut down and the bladder wet weight was measured. After Haematoxylin- Iraq red (HE) staining, the changes of the shapes about detrusor of bladder of diabetic rats were observed under the light microscope. On the basis of these results diabetic model group's rats'DC was formed. The expression of NGF in the cell of detrusor of bladder and the neurons of L6-S1's DRG were detected by immunohistochemistry. Under the light microscope the result was observed, it was positive result in the urinary bladder detrusor cell or the neurons of L6-S1's DRG when yellowish brown pellet emerges. Each slice was chosen 8-10 fields of vision and registered 100 cells morphological change under the high objective lens(×400). The standard of judgment: masculine cells<10% was negative expression (-), masculine cells≥10% and <30% was feeble masculine expression(+), masculine cells≥30% and <60% was middle dimensional masculine expression(++), masculine cells≥60% was powerful masculine expression(+++). The datas were analyzed by t and t' and ranked data'rank sum test with SPSS 13.0 system software and the result was mean±standard deviation (Mean±Std.).Result: The model after eight weeks was successfully set up, the blood glucose in the diabetic model group'rats was obviously higher than that in the normal control group'rats, and the result was (30.9±3.3mmol/L) vs (4.8±0.3mmol/L) (t'= 35.260, P<0.05). The body weight of the diabetic model group'rats grew slower than that of the normal control group'rats, and the result was (248.05±15.52g) vs (322.80±15.92g) (t=13.949, P<0.0005). The twenty four hours'voiding volume of the rats in the metabolic cage was determined: the voiding volume of the diabetic model group'rats was obviously greater than that of the normal control group'rats, and the result was (88.31±7.22ml) vs (20.93±2.90ml) (t'=37.853, P<0.05). The bladder wet weight of the diabetic model group'rats was obviously heavier than that of the normal control group'rats, and the result was (171.15±6.08mg) vs (135.53±5.33mg) (t=18.059, P<0.0005). We observed the rats with naked eye. The rats in diabetic model was observed in whole-body, which were shown that their skin and capill lost gloss and they drank more water and ate more food and urinated more urine and excreted more thin excrement. Under the light microscope detrusor structure of two group of rat was observed. Normal control group: Transitional epithelium cell arrangement was neat, membrana propria was integrity. The size and shape of detrusor cell was homogeneous and muscle fiber was arranged in order. Structure of muscle bundles was close and the gap was filled by phoroplast tissue. Under the mucous membrane and the intermuscular nerve plexus was clear. Diabetic model group: Transitional epithelium cell arrangement was not neat. Membrana propria was hyperemia and dropsy and size of detrusor muscle cell was large and the shape was diverse. Muscle fiber was arranged disorder and out of order and cross of muscle fiber was full and space between the muscle fiber was enlarged obviously. The collagen and fiber structure was proliferated and oxyphil has proliferated in the submucosa. Small vessels hyperemia between muscle bundles. Based on these results that diabetic model group's rats'DC was formed. The results of the immunohistochemistry were that: NGF was mainly distributed in the endochylema of the detrusor muscle's cells in the Wall of urinary bladder and was expressed a small quantity in the transitional epithelium and the lamina propria. NGF was also mainly distributed in the endochylema of the neurons of L6-S1's DRG. The datas were dealed with statistics. The result of ranked data'rank sum test was shown that the expressed levels of NGF in the bladder and L6-S1's DRG in the diabetic modal group's rats were both significantly lower than that in the normal control group's rats, and the consequence were (μ=3.1499, Phalf<0.001) and (μ=3.1039, Phalf<0.001). Conclusion: The expressed levels of NGF were both reduced in the bladder and L6-S1's DRG of the type I diabetic model rats with STZ injection. The expressed change of NGF played an important role in the development of DC.
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