The Detection Of Human Papillomavirus DNA In Squamous Cell Carcinoma Of The Head And Neck By PCR

Objective To investigate the potential correlation between the infection of human papillomavirus (HPV) and the oncogenesis of squamous cell carcinoma of the head and neck (SCCHN). Methods The paraffm-embeded tissues from the patients with SCCHN were detected by PCR, which used general primers Gp5VGp6* system and type-specific primers HPV16/18 system to amplify the HPV DNA extracted from these specimens. Results The results indicated that HPV DNA were founded in 15(20.5%) of 73 squamous cell carcinoma of the head and neck( SCCHN), in 10(18.9%) of 53 Nasopharyngeal carcinoma (NPC), respectively. No HPV DNA was found in the chronic inflammatory mucosa lesions. The HPV 16 was the main type identified in the most of positive cases, followed by the HPV18. Conclusions The results implicated that the HPV prevalence may correlate with SCCHN. However, HPV status was not correlative with sex, aging, tumor site and degree of differentiation.