Correlation Between The Polymorphism Of VWF Gene Thr789Ala And The Level Of VWF With Coronary Artery Atherosclerotic Disease

Background With the improvement of socioeconomic status and the change of lifestyle in human being, especially in China, morbidity and mortality or the ratio of disability of coronary artery disease have been widely increasing. The role of gene mutation, especially in clotting system have been paid more attention, for example, the association between the vWF gene Thr789Ala polymorphism and coronary artery disease in IDDM. But up to now, there has been few reports in china. The objective of the study is to reveal the association between the Thr789Ala vWF gene polymorphism and coronary artery disease and the level of vWF in plasma in Han ethics of Tianjin city in China and the role of vWF in the development of coronary artery disease.Methods C AG were performed in all the observed and the subjects of the study were divided into two groups: CHD group and non-CHD group. The vWF level was measured by immune turbidimetry and the vWF Thr789Ala polymorphism was evaluated by RFLP. The level of biologic parameters such as fibrinogen, lipoprotein was available from biochemical examination. The relationships between cardiovascular risk factors and coronary artery disease were investigated.Results (1) The vWF level of CHD group was 182.49+44.74, non-CHD group 169.18+38.54 (P=0.028), respectively. But in paired group the significant difference dispears when precluding the effect of the relative factors, CHD group was 174.44 +35.09; non-CHD group 174.17+42.47(P=0.979). (2) Thedistribution of the vWF Thr789Ala polymorphism was Thr/Thr (homoygote for Thr789): Thr/Ala (heterozygote for Thr789 ) genotype 102 ( 89.5%): 12( 10.5%) in CHD group; Thr/Thr: Thr/Ala 75 (86.2%): 12 (13.8%) in non-CHD group, respectively; Ala789 homoygote (Ala/Ala) was absent in two groups. There was no significant difference between the two genotypes in two groups(P=0.950). In the present population A and G allefrequency is 94.54% and 5.46% in CHD group; 94.44% and 5.56% in non-CHD group. In the two groups, the levels of vWF were influenced significantly by essential hypertension, higher lipoprotein and higher fibronogen, not by vWF Thr789 polymorphism, body mass index, and so on. Multi-Logistic regression analysis reveal that higher vWF and heterozygote of Thr789 genotype is not the independent risk factor of coronary artery disease.Cone I us i on The present data suggests that (1) There was no significant difference of the vWF level between the CHD and non-CHD groups when precluding the the effect of relative factors, and high vWF level is not the independent risk factor of CHD, maybe dependent. (2) The heterozygote for Thr789 is not the independent risk factor for CHD in Tianjin city Han ethics group, it is a lower ratio in all, (3) vWF level in plasma does not associate with vWF mutation in Thr789, and maybe influenced by essential hypretension, higher lipoprotein and higher fibrinogen independently, and so on.