| Androgenetic alopecia( abbreviate to AA) is the most common form of baldness observed in human beings; its many synonyms include male pattern baldness, seborrheic alopecia and diffusion alopecia. It follows a characteristic pattern with the hair initially receding bilaterally backwards from the frontal-temporal region after the puberty. Statistics data shows that the prevalence rate of Han people reaches 30.2%m. With the rise of living standard, people become to think highly of the treatment of AA. The metabolism of androgen in hair follicle is the focus and difficult point in recent AA study. The primary direction of AA study is to investigate the expression of androgen and androgen receptors(AR) in target organ, to detect the competing AR inhibitor . In order to investigate the correlation between the syndrome classified by Traditional Chinese Medicine (TCM) and androgen receptors N hair trace element in androgenetic alopecia, we used immunohistochemistry method and ICP-AES method.Try hard to quantitate the differential diagnosis of TCM from microcosmos, find out the parenchyma of the syndrome classified by TCM: syndrome of appetite-wet heat and syndrome of deficiency in liver and kidney, investigate the regulation mechanism of endocrine in TCM, and provid a quantitative index and a new method relevant to future AA treatment. 1. Literature SudyThe author has reviewed a lot of TCM literatures and the articles of western medicine about AA. At present, though TCM has gained some obvious achievements in studying AA, there are still some questions to be answered such as the following: 甌here are many clinical reports of treating AA with TCM nowadays. Most of them are selection of treatment based on the differential diagnosis. Which is th basic approach to treat diseases in TCM. Since the selection of treatment based on the differential diagnosis in TCM has not yet been standardized, it is very difficult to evaluate the effectiveness of treatment and popularized it. Hence it is critical to standardize differential diagnosis and study AA objectively; 〦ven though TCM has4made progress in removing dandruff and stopping hair loss, it is still short in hair growth and its theory study; ㎜ong-term effect of current AA treatment is not satisfied. Some patients lose hair again after withdrawal between half year to a year; @It is also deficient in the regulation mechanism research of endocrine in TCM. 2.Experiment Study(1)166 eligible cases were collected and categorized into two groups. The two groups are respectively syndrome of appetite-wet heat and syndrome of deficiency in liver and kidney. We use ICP-AES method to detect the six trace element (Zinc(Zn), Iron(Fe), Calcium (Ca), Cuprum (Cu), Manganese (Mn), and Plumbum (Pb)) of patient's hair.(2) In order to investigate the correlation between the syndrome classified by TCM and AR in AA, AA cases were classified as syndrome of appetite-wet heat and syndrome of deficiency in liver and kidney. 80 eligible AA cases and 32 normal controlled case were collected. We detected their AR level from balding area and non-balding area with immunohistochemistry method. 3. Result (D Trace element of hairWe found the trace elements Zn* Fe, Cu> Ca and Mn in AA patients' hair was significantly lower than the normal value, while Pb was significantly higher than the normal value. Zn value and Zn/Cu odds were significantly different between syndrome of appetite-wet heat and syndrome of deficiency in liver and kidney (P<0.01). (2) The distribution of ARARs were present in granular cell layer, stratum spinosum, basal layer, fibroblasts, blood vessel endothelial cells, nerves, arrector pilli muscles, sweat glands, sebaceous glands, outer root sheath, and hair papilla, they were all nuclei staining. ARs were absent from cuticular layer, adipose tissue, inner root sheath, hair shaft, and hair matrix. ARs were present in dermal papillae during anagen and telogen stages of hair growth cycle. ?Observation of morphologThe hair papillas from balding area were smaller than...
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