Effect Of Ganoderma Applanatum Polysaccharides On P53 Gene Expression In Mouse HepA Tumour

AIM: To study the influence of GAPSGF on p53 gene expression in HepA tissue. This will help to complete the mechanism of antitumour effect of GAPSGF. METHODS: ﹖he ratio of antitumour: Weigh the intact tumours of each group and calculate the ratio. ㎡bserve the effect of GAPSGF on p53 gene expression by the method of ELISA. ㎡bserve the effect of GAPSGF on the serum protein components by SDS-PAGE. (4) Observe the influence of GAPSGF on the tumour tissue protein components by means of SDS-PAGE. 〤onclude the influence of GAPSGF on PCD by DAGE. RESULTS: 〤ompared to Negative Control Group, both GAPSGF Group and Positive Control Group have notable difference (p<0. 05) , and the two , comparing to each other, have no difference (p>0. 05) .?There is much obvious difference between Negative Control Group and Norm Control Group (p<0. 01); Comparing GAPSGF Group and Positive Control Group with Negative Control Group, they have also much significant difference (p<0. 01) and they are similar to Norm Control Group (p>0. 05) , there is no difference between GAPSGF Group and Positive Control Group(p>0. 05). 〤ompared to Norm Control Group, Negative Control Group is understained at the band of Mr 115kd in the serum SDS-PAGE map, both GAPSGF Group and Positive Group can make it return. (4)In the tumour tissue SDS-PAGE map, a: In comparison to Norm Control Group, Negative Control Group is dark-dried ; GAPSGF Group can make it light, but Positive Control Group has no change to it at the band of 65kd. b: There is a more hyperchromatic band in Norm Control Group than Negative Control Group at the band of Mr 53. 7kd, GAPSGF can make it understained and similar to Norm Control Group. Positive Control Group can also lighten it, but can' t resume it. c: At the band of Mr 12. 6kd, there is no notable difference among Norm Control GroupN Negative Control Group and Positive Control Group, but it is deep-dried in GAPSGF Group. ㊣n the DAGE map, a single$f GF jft/h m HepA ?p53hyperchromatic broad band is showed in Norm Control Group and Negative Control Group, but staircase bands , which are spaced by the euplidy of 200bp , are showed in GAPSGF Group and Positive Control Group. CONCLUSIONS: 〨APSGF has obvious antitumour effect on HepA tumour. 〨APSGF can obviously attenuate the expression of p53 mutation gene in HepA tumour and make P53 similar to Norm Control Group . (3) GAPSGF can resume the abnormal serum protein component. can resume some tumour abnormal tissue protein components. can probably accelerate HepA apoptosis.