| Objective: To investigate Helicobacter pylori (HP) pathogenesis infection in family and to analyze the genetype of Hp. To observate HP transmission and the effection of intrafamilial infection on children. In order to offer experimental data to prevent HP infection.Methods: Twenty families of HP positive were detecte by nested PCR. The genetype of HP were distinguished by PCR directly. In this study, four pairs of genetype primers for PCR were designed. Among them, the first pair is for cagA genetype , the second pair is for vac Ami genetype, the third pair is for vacAm2 genetype and the last pair is for vac As 1 genetype. The experiment showed that the four primers can be used effectively for amplification of aimed gene. The products of PCR was detected on the Agarose gel electrophoresis and photographed. The results were analyzed by using Chi-Square Test and Fish's Exact Test.Results: 1. HP pathogene positive infection rate in samples was 100% (60/60) 20 families of HP-positive were all infected with one or more kind of HP genetype (cagA, vac Ami, vacAm2, vac As 1 genetype). 2. The cagA genetype HP-positive infection rate in adults and kids was 45% (27/60), 55% (11/20) respectively, the difference between both groupswas no significant (P>0.05). 3. The vacAml, vacAm2, vac As 1 genetype Hp-positive infection samples in adults were 29/40, 23/40, 28/40, respectively and kids were 12/20, 9/20, 11/20 respectively, the difference between both adult and kid groups was no significant (P>0.05). 4. Of all the selected samples, the cagA genetype, vacAml, vacAm2, and vac As 1 genetype infection rate was 63.3%, 68.3%, 53.3%, 65% respectively. The difference of four genetypes was no significant (P>0.05).Conclusions: (1) HP pathogene infection exists in saliva, HP pathogene infection rate was 100% in this study. (2) The infection rate of same genetype HP strain was 100% in one family. (3) The main causes of children early infection was HP pathogene transmission in family. |
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