| Radix notoginseng is a rare traditional Chinese herb widely used in the oriental world with the indication of promoting blood circulation to remove blood stasis and stopping bleeding, alleviating pain. It is an important traditional drug for various bleeding or blood stasis and tranma. The pharmacological research was concentrated on the saponins and many activity of them have been tested. Radix notoginseng contains a mixture of structurally similar saponins which represents the main active contituents of it. The most saponins in it are classed into dammarane type 4 rings triterpenes, hydrolyzates of which are panaxadiol and panaxatriol. Notoginseng contains about 8~12% by weight of saponins which comprise of ginsenosides such as Rg1> R^ Rb1> Rb2, Ras Rd, Re and notoginsenosides such as R,> R2. Notoginseng is usually used as an ingredient in a traditional Chinese complex prescription. The analysis of saponins is an important method to evaluate the quality of notoginseng and its Chinese medicine preparations. Denshen Dropping Pill in complex prescription is composed of Radix Salviae Miltiorrhizae, Radix Notoginseng and Borneolum Syntheticum, manufactured by modern pharmaceutics. It possesses the pharmacological action of promoting blood circulation to dissipate blood stasis, regulating Qi and relieving pain. In clinical therapy it is an useful drug for cardiovascular diseases.High performance liquid chromatography-evaparative light scattering detector(HPLC/ELSD) is a new method to analyse saponins. ELSD is a universal detector with superiority to determining chemical constituents without ultraviolet absorption structure. In this paper a HPLC/ELSD method has been established to assay ginsenoside Rg1- Rb1 and notoginsenoside R, in Radix Notoginseng and its preparation. In sample pretreament Solid Phase Extract(SPE) is used to purify the aqueous extract. This work can be an useful example for analysis and quality controll of preparations containing notoginseng. 1. Assay of ginsenoside Rg1> Rb1 and notoginsenoside R, in Radix Notoginseng A method has been established to assay Ginsenoside Rg1 and Rb1,Notoginsenoside R, in Radix Notoginseng by HPLC/ELSD with Solid Phase Extraction. The three saponins can be separated from each other and determinated on a Hypersil NH2 column(200mmX4.0mm15p m) with the mobile phase acetonitrile-isopropyl alcohol-10mM ammonium acetate buffersolution(pH 5.0)(75:20:5);flow rate 0.6mL/min. The linear range was from 1.0 to 10.0|jg for ginsenoside Rg1 and Rb1,notoginsenoside R^The average recoveris for them were 95.5~102.5%.The RSD for inter-day and intra-day were less than 2% and 4%. The method is concise and accurate.2. Assay of Ginsenoside Rg1and Rb1,Notoginsenoside R, in Danshen Dropping Pill by HPLC/ELSDA method has been established for purifying and assay. Ginsenoside Rg1 and Rb1, notoginsenoside R, in Danshen Dropping Pill were derivatizid with SPE, then assayed by HPLC/ELSD.The chromatogrphic and ELSD conditions all are the same as above, he linear range was from 1.0 to 10.Dug for ginsenoside Rg1 and Rb1, notoginsenoside R^ The average recoveris for them were 95.3~100.4%.The RSD for them for inter-day and intra-day were less than 2% and 4%.The main constituents in Danshen Dropping Pill are water soluble and the object analytes are polar compounds. How to remove the interference is the first question to solute in sample pretreament. To purify the sample with SPE we can obtain the good performance with superiority of using fewer sample dosage and solvent, concise operation.3. Study on the re-usability of the SPE cartridgesWe took the same sample and processed them in 8 times in the same SPE cartridge. The peak area of ginsenoside Rb1 was determined. The data of peak area and the re-using times of cartridge was processed by one-way ANOVA. The result showes that in 8-time experiments the efficacy SPE cartridge has not significant different. A considerable cost-saving may be achieved by re-using the SPE cartridges.
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