| Wilms' tumor is one of the most common abdominal malignant tumor in children.In 1899,German doctor Wilms reported this disease for the first time,so it was named after his name.Because it is generated from rear renal embryo in embryogenesis and the tumor are consisted of cells that looking like nephrogenic cellsjt is also called nephroblastoma.In children ,its incidence is only second to that of neuroblastoma in some countries but is a little higher than the laters in china.The tumor usually generate in the first five years,especially at the age of 2 - 4years.lt often generated with congenital malformation,such as congenital lacking of iris,congenital limbs hypertrophy in one side.The tumor generate from indifferential renal embryo and can form every part of nephridium.This mixed malignant tumor is consisted of mesenchymal tissue and epithelial tissue,andnowadays its main prognosis factors are the histological strutcure,age,the totally excision of original tumor, metastasis and two sides of tumor. We still don't find specific diagnostic marker in nephroblastoma now.By means of immunohistochemical techniques, to investigate the expression of endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF), and to study their relationships in nephroblastoma and the their role in the angiogenesis in nephroblastoma,to reveal the relationships among them and the biogical characters of this tumor . All these will provide people with new thoughts and basis to prevent and treat cancer.Materials and methods: we have collected 50 children's nephroblastoma specimens from surgical resections, and all sections were formalin-fixed and paraffin embedded. The patients, aged from 4 months to 15 years , average 5.44+4.73 years, 32 males and 18 females, were not performed any radiotherapy and chemotherapy before operation.Their HE staining sections were affirmed and histopathologic types were classified. Their clinical stage were classified according to the condition during operation and the pathologic diagnosis after operation (by NWTS-3 Criteria). Among them ,30 patients had stage I-II , 20 stage III-IV . 40 patients had FH (favorable histology) type tumor, 10 UH (unfavorable histology) type . The expression of MVD, eNOS andVEGF in 50 patients with nephroblastoma were studied retrospectively by using immunohistochemical staining (SABC). The immunohistochemical staining of eNOS, VEGF were assessed according to this criterion: those having positive staining in less than ten percent of tumor cells were graded as "- " ,more than ten percent as "+". MVD was assessed by the mean of blood vessel quantity of five"hot spof'under 400 x microscope. Statistical analysis was executed by SPSS 10.0 software. P values less than 0.05 were considered significant.Results: l.The MVD in 10 patients (UH type) was 85.92 + 14.36 which was significantly higher than that in 40 patients (FH type) was 50.57+ 13.01(PO.05); The MVD in 20 patients (III-IV stage) was 70.39+17.59 which was significantly higher than that in 40 patients ( I-II stage) was 49.14+15.70 (PO.05). 2.The expression rate of VEGF was 80.00% in patients (III-IV stage) which was significantly higher than that in patients ( I-II stage), (P<0.05). The expression rate of VEGF was 36.67% in patients ( I-II stage) .The expression rate of VEGF was 65.00% in FH type while it was 60.00% in UH type (P>0.05).The MVD in VEGF positive group was 65.70+16.88 and was higher than that of VEGF negative group (PO.05). 3.The expression rate of eNOS was 70.00% in patients(UH type) which was significantly higher than that in patients(FH type) (P<0.05) . The expression rate of eNOS was 27.50 % in patients(FH type). The expression rate ofeNOS was 60.00% in III-IV stage and expression rate of eNOS while it was 20.00% in I-II stage, (P<0.05).The MVD in eNOS positive group was 73.13 + 19.38 and was higher than that of eNOS negative group, (P<0.05). 4.There was no relation between the expression of VEGF and the expression of eNOS in nephroblastoma. 5. The expression...
|
PDF Full Text Request