| Intracerebral hemorrhage is one of frequently occurring disease innervous system. It has higher the rates of death and cripple than other disease as the focus of hemorrhage constricts brain tissue and causes increased intracranial pressure, and it is important leading to secondary cerebral lesion, such as ischemia, edema of perihematomal brain regions and the change of the cell biochemical. Recent studies have confirmed that cytokine is involved in the pathogenesis of cerebral hemorrhage. Basic fibroblast growth factor(bFGF), vascular endothelial growth factor(VEGF) and tumor necrosis factor(TNF- a ) belong to cytokine. It is an aid to clarify the mechanism of the disease to study bFGF,VEGFand TNF- a change after Intracerebralhemorrhage with moleclar biological technology. In nervous system, positive expression of bFGF and TNF-α is neurons and astrocytes, and positive expression of VEGF is vascular endothelial cells. The content of bFGF protein in brain regions different as time change after brain injures. There was no any literature about detecting the expresion of bFGF, VEGF and TNF-α protein in the perihematomal brain regions after intracerebral hemorrhage. Using immunohistochemical technique with the expression of bFGF,VEGF and TNF-α in the perihematomal brain regions is observed in order to research the pathology mechanism of Intracerebral hemorrhage . The purpose of this study is to provide theoretical basis for the treatment of intracerebral hemorrhage.Materials and methods: (1) Dispart 40 healthy big ear white rabbits to 4 groups randomly. Group A(10 rabbits) is normal comparative group, group B, C and D(l() rabbits per group ) is experimental objects. Group B are 1 day after Intracerebral hemorrhage, when group C 3 days, groupD 7 days. Models of Intracerebral hemorrhage are made by injecting its self-blood into the cerebrums of each rabbit. The puncture is at 0.6cm on the right fromthe middle line and O.lcm in front of the sutura coronalis, with 45 angle-right and down direction from the skin, with a self-made brain-piercing needle, with a depth of 1.3cm~1.4cm. Inject 0.2ml blood for the first time, and 0.4ml 30min later, with the needle staying in for 30 min, so as to prevent the uncongealed blood flowing out from the puncture hole. (2) Behead the three groups of rabbits respectively on the 1, 3 and 7 days after modleinject 10% neutral formaldehyde liquid into the brain through carotid artery. Take out the perihematomal brain tissues and embed them in parffin and make immnohisto-chemical section. (3) Test the level of expressions of bFGF,VEGF and TNF-α in the perihematomal brain tissues by the method of immunohistochemical SP. (4) Using international currency statistics software SAS6.12, we deal with the date processing. Statistical method is analysis of variance (ANOVA) (test q and test q') and significant level a=0.05.Results: (1) bFGF, VEGF and TNF-α in nerve cell of the comparative group expression level lower than the experiment groups. Positive cells of bFGF: Group A 8.60 ± 0.99; Group B 15.04 ± 1.43; Group C 29.52 ± 2.03; Group D 18.56 ± 1.36 Every experimentalgroup compare with the comparative group has significant difference(p<0.05), so as among the expriment groups(P<0.05). Group B is the lowest, group C is the highest, group D is in the middle. (2) Positive cells of VEGF: Group A 3.02 ± 0.82; Group B 6.38 ± 1.02; Group C 11.26 ± 1.65; Group D 18.64 ± 1.58;. Every experimental group compare with the comparative group has significant difference(p<0.05), so as among the expriment groups (P<0.05). Group B is the lowest,group D is the highest, group C is in the middle. (3) Positive cells of TNF-a : Group A 5.44 ±1.07; Group B 31.78 ± 2.30; Group C 16.48 ± 2.10; Group D 10.35 ± 1.50; Every experimental group compare with the comparative group has significant difference(p<0.05), so as among the expriment groups (P<0.05). Group D is the lowest, group B is the highest, group C is in the middleConclusions: (1) bFGF, VEGF and TNF-α of every exprimen...
|
PDF Full Text Request