Effects Of BFGF Protections On Brain After Intracerebral Hemorrhage In Rats

1.Effect of bFGF on the apoptosis of nerve cells in rats after intracerebral hemorrhageAIM:To observe the effect of basic fibroblast growth factor(bFGF)on neuronal apoptosis after intracerebral hemorrhage(ICH)in rats. METHODS:The rat models of experimental ICH were established by injection of collagenaseⅦinto internal capsule of cerebrum.The enrolled 54 Wistar rats were randomly divided into of model group,normal saline(NS)control group and bFGF treatment group.Each group subdivided into one day,theree days and seven days time points.the apoptotic number of nerve cells in cerebral cortex and hippocampus were measured with terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL),and counted the number of neuronal apoptosis cells under the high power microscope.RESULTS:①At day 3 apoptotic numbers of cortex nerve cells in the bFGF treatment group was less than that in the NS control group(p＜0.05).②At day 7 apoptotic numbers of cortex nerve cells in the bFGF treatment group was less than that in the NS control group(p＜0.01).③At day 7 apoptotic numbers of nerve cells in hippocampus of the bFGF treatment group was less than that in the NS control group(p＜0.05).CONCLUSION:The bFGF can inhibit and reduce the apoptosis of nerve cell after ICH.2.Regulatory effects of bFGF on the expressions of Bax and Bcl-2 genes in the perihematoma and hippocampus tissues after ICH in ratsAIM:To observe the effects of bFGF on the expressions of Bax and Bcl-2 genes in the perihematoma and hippocampus tissues in rats with ICH,and explore the regulatory effect of neurotrophic factor on nerve cell apoptosis.METHODS:ICH models were established by injection of collagenaseⅦinto internal capsule of cerebrum.The enrolled 72 Wistar rats were randomly divided into of model group,NS control group and bFGF treatment group.Each group subdivided into 1 day,3 days and 7 days time points,respectively to harvest the perihematoma and hippocampus tissues under anesthesia.The expression levels of Bax and Bcl-2 genes were detected by RT-PCR.RESULTS:①Bax mRNA expression around perihematoma tissue:After 3 and 7 days intervention, the expression level of Bax gene of the bFGF group were significantly decreased compared with the NS control group(p＜0.01 or p＜0.05).②Bcl-2 mRNA expression around perihematoma tissue:After 3 and 7 days intervention,the expression level of Bcl-2 gene of the bFGF group were significantly increased compared with the NS control group (p＜0.01 or p＜0.05).③Bax mRNA expression in hippocampus tissue:After 3 and 7 days intervention,the expression level of Bax gene of the bFGF group were significantly decreased compared with the NS control group(p＜0.05).④Bcl-2 mRNA expression in hippocampus tissue: After 3 and 7 days intervention,the expression level of Bcl-2 gene of the bFGF group were significantly increased compared with the NS control group(p＜0.05).CONCLUSION:bFGF could regulate the apoptosis-related genes,increase the expression of Bcl-2 gene and reduce the expression of Bax gene around perihematoma and hippocampus tissue.3.Effects of bFGF on the SOD activity and MDA content in the perihematoma and hippocampus tissues after ICH in rats AIM.To investigate the effect of bFGF on the activity of superoxide dismutase(SOD)and content malondialdehyde(MDA)in the perihematoma and hippocampus tissues in rats with ICH.METHODS: ICH models were established by injection of collagenaseⅦinto internal capsule of cerebrum.The enrolled 90 Wistar rats were randomly divided into of model group,NS control group and bFGF treatment group.Each group subdivided into 1 day,3 days and 7 days time points,respectively to harvest the perihematoma and hippocampus tissues under anesthesia.The activity of SOD and content of MDA in perihematoma and hippocampus tissues were detected.RESULTS:①After 3 and 7 days intervention, SOD activities in the around perihematoma tissue of the bFGF group were significantly increased compared with the NS control group respectively (p＜0.05).but MDA contents in the around perihematoma tissue of the bFGF group were significantly decreased compared with the NS control group respectively(p＜0.05).②After 7 days intervention,SOD activities in hippocampus tissue of the bFGF group were significantly increased compared with the NS control group(p＜0.05).but MDA contents in hippocampus tissue of the bFGF group were significantly decreased compared with the NS control group(p＜0.05).CONCLUSION:bFGF possess the reliable function of eliminating free radicals,decrease MDA content and increase activities of SOD.