| Human astrocyte tumors are the most those of neuropithelial tissue. It is reported that it account for 13%-26% in intracranial tumors as well as 21.2%-51.6% in gliomas. Nowadays, The main methods of treatment are still operation, radioneurosurgery and chemotherapy. Although it can get better effect in short period, the further effect still cannot be satisfying. Presently, Etiology of the astrocyte tumors still hasn't been total knowledge and we cannot heal it. In multiple cell tissue, they are very complex communication in cell interior and between cell and cell. Every cell adjusts accurately its action to satisfy-5-tissue needs. The adjustment of cell in interior and betweens are finished by a large numbers of signal molecules and its acceptor. Adjusting and according multiple cells compose a complex organism. Cell cycle of multiplication includes Gl, S, G2 and M periods. Multiplication process orderly. They are controlled by many kinds factors. There are two critical points in cell cycle whose role is limit velocity to cell multiplication. They, beyond control, will lead to tumor appearance. We could project anti-cancer drag to hamper cancer appearance caused by growth factors, knowledge of Gene mutation as well as interactive and intercommunication mechanism between cells will,at last, make us defeat cancers. So, recently, it is one of the most active research regions.p27Klpl, cyclin dependent kinase inhibitor, is one of Cip/Kip family, plays a important role during cell cycle. That PCNA is a market of manifesting cell multiplication is an acid nuclear protein. As a assistant factor of DNA synthesis, it attend directly synthesis. So, it can reflect effectually capability of cell multiplication.The study observes expression p27Klpl and PCNA in human astrocyte tumors and correspondence between them. Further study p27Klpl's roles of human astrocyte tumors in itsappearance and development, and initiatively estimate its diagnosis signification in human astrocyte tumors. It is a basis for study further p27Klpl in human astrocyte tumors's appearance and development, and as a theory basis for identifying diagnosis and estimating prognosis as well as genetic treatment.Materials and methods:1. Specimens: collection of 60 human astrocyte tumors was cut by the second affiliated hospital from 19.95 to 2001 includes 20 astrocyte tumors, 20 anaplasia astrocyte tumors as well as 20 glioblastoma. In addition to them, 20 non-human astrocyte tumors are been as normal comparative group. All specimens was fixated by 10% formalin, buried by wax, then split 5 um sections. Reagents: (1) monoclonal mouse anti-P27 protein (work type) and monoclonal mouse anti-PCNA protein (work type) was bought from maxim company (2) Histostain -SP kits was bought from maxim company. 2. Groups: group A: non-human astrocyte tumors. Group B: human astrocyte tumors. Groups C: anaplasia astrocyte tumors. Groups D: glioblastoma. Methods: test the level of expression of p27Klpl and PCNA in human astrocyte tumors and non-human astrocyte tumors with immunohistochemical SP. using international currency statistics software spss 10.0, we deal with the data, analysis of variance(ANOVA) is used between sample means, test q' between experimental group and opposition one, test q between two means. Relation between two variances is analyzed by correlation, significant level is a = 0.05. Experimental data are expressed by X+S. Results:(1) Positive cells of protein p27Klpl exhibit cell nuclear dyed brown. Positive cells distribute widely and lower expression of p27Klpl, higher malignancy degree. Between non-human astrocyte tumors and every kind of human astrocyte tumors, expression of protein p27Klpl LI shows that there are a remarkable difference between non-human astrocyte tumors and every kind of human astrocyte tumors (p<0.05). With improvement of pathologic glass, expression of p27Klpl drops. In addition to between anaplasia astrocyte tumors and glioblastoma, there are remarkable differences between other groups.(2) P...
|
PDF Full Text Request