A Preliminary Study On Angiogenesis In Myelodysplastic Syndromes

Objectives:This study was purposed to investigate the angiogenesis status in myelodysplastic syndrome,and understand its effects on the pathogenesis of this disease. We compared the difference of S-VEGF and MVD between patients and normal persons,and analyzed the relationship with age,peripheral blood counts,marrow blast percentage,and ALIP,so as to find some of abnormal angiogenesis in MDS,and perhaps it would be as a new evidence for anti-angiogenesis therapy.Methods:We collected the blood from 20 patients with myelodysplastic syndrome and 20 normal persons respectively. The level of S-VEGF was measured by ELISA,and the microvessel density (MVD) of marrow biopsy specimen was examined with streptavidin-biotin immunoperoxidase method. The counts of MVD were evaluated with image analysis. Statistic was analyzed with SPSS 10.0 software.Results:1) The level of S-VEGF and the counts of MVD in the MDS patients were higher than those of normal persons (p<0.05). Except for MDS/MPD cases,there were no significant differences of S-VEGF and MVD in the other MDS subtypes. 2) S-VEGF was positively related with MVD (P<0.05). 3) S-VEGF and MVD are closely related with age,peripheral bloodcounts,marrow blast percentage,and suggest that MDS in progressive status,they may be as prognostic factors.Conclusions:This study shows that S-VEGF and MVD are closely related with angiogenesis and disease progressive status in myelodysplastic syndrome patients,and also offers a new therapeutic strategy for MDS. Factor VIII is a better marker of immunohistochemical technique to evaluate marrow angiogenesis.