Studies Of The Differentiation Effect Induced By BPO-L On The Human Lung Adenocarcinoma A549 Cells

In this study, we used BPO-L, together with a HMBA, curcumin as control and their combination to treat the human adenocarinoma A549 cells for investigating the biological effects and them antiumor mechanisms of BPO-L by the methods of biochemistry, cell biology and immune biology.The results revealed that the proliferation of A549 were effectively inhibited after being treated with 0. Img/mL BPO-L, the rate of cell growth and mitotic index inhibitory were 39. 5% and 28.95% respectively, the cell was apparently changed. With flow cytometry, it has been found that G0/G, phase markedly increased, whereas S and G2+M phases decreased. Light and electron transmission microscopy showed that A549 cells which treat with BPO-L, HMBA or their combination, had undergone restorational alteration in morphology and ultra structure changes similar to those normal cells as follow: the cell turned to be flat, the nucleo-cytoplasmic ratio lessen and nuclear shape became regular, the euchromatin increased while heterchromatin decreased in nucleus. In the cytoplasm, golgi complex turned to be well-developed and typical, rough endoplasmic reticulum increased, polyribosome decreased while free ribosome increased. Themorphology and ultra structure of A549 cells which treated with curcumin were similar as typical apoptosis, while those which treated with the combination with BPO-2 and curcumin were not change apparently. Furthermore, the immunocytochemical assay revealed that the expression proteins of oncogene mutant p53, c-myc and Bcl~2 were downrcgulated but the protein show the protein products of tumor suppressor gene p!6, p21 WAFI/CIP1 and Rb were increased after the treatement of BPO-L, HMBA and their combination. All of these results showed that the BPO-L could not only effectively inhibit the proliferation of the human lung adenocarcinoma A549 cells, arrested the cells in G0/G, phase, as HMBA, but also reverse cell malignant phenotypes and regulate and interfere the expression of oncogene and tumor suppressor gene correlative with lung cancer. It could be conclude that BPO-L has the induced-differentiation effects on the human lung adenocacinoma A549 cells.