Effects Of Recombinant Growth Hormone On Serum Lipids And Atherosclerosis Of Rabbits

Background: Hypercholesterolemia is a common clinical metabolic and/or genetic disorder that promotes functional and structural vascular wall injury. It is the most important risk factor of coronary artery disease (CAD). Low density lipoprotein Cholesterol(LDL) is synthesised with very-low- density lipoprotein Cholesterol ( combined with Cholesterol and triglycerides), and is analysed after connected with its receptor.Many studies have supported that TC and LDL-C and ApoB could accelerate atherosclerosis.It is recognized that TC was the key risk factor,so the measure to decrease TC is the basis therapy step.GH is downside with age,s arise in human body and the incidence of atherosclerosis increases with age,s up going.De boer finded that TC, LDL-C and ApoB were higher in GHD than in normal human.Leese and Hansson finded that GH could decrease TC and LDL-C when they treated GHD with GH. Recently, much attention has been focussed on the effects of GH to increase cardiac mass suggesting its possible application in the treatment of chronic heart failure,but the function of GH on serum lipids and atherosclerosis are unknown.Objective: To study serum lipid and atherosclerosis are wether associated with GH,and the expression of hepatic LDL receptor mRNA or not after GH intervention.Method:1.4-month-old male rabbits weighting 2.2±0.3kg were housed under high-cholesterol diet with GH intervention.2. Serum lipids,NO and ET were measured after 3 monthes.3. Rabbits aortic strips were observed under microcope and electrimicrocope to check the atherosclerotic pathological changes.4. Expression of LDL receptor mRNA was assessed by RT-PCR.Results:1.GH could decrease TC,LDL-C,Lpa and ApoB,but not the TG.2. The endothelial cells realaxation function in GH group was better than in HC group.3. The rate of recurrence of Atherosclerosis and fatty liver in HC group was higher than other groups.4. The expression of LDL receptor mRNA was accelerated in GH group.Conclusion:1. GH could decrease TC,LDL-C and Lpa in serum of ribitts under high-cholesterol diet,and could improve the injury of endothelial cells function.2. One of the effects of GH was the acceleration of the expression of LDL receptor mRNA.