Ghr Gene Exon 3 Deletion Polymorphism Study On The Correlation Between Nonalcoholic Steatohepatitis And

Objective: The aim of this study was to identify carriers of GHRd3 variant in Japanese population and inquire the role of functional polymorphism of growth hormone receptor gene as a risk for developing NASH.Methods: Blood samples of 103 patients with biopsy-proven NASH and 150 healthy volunteers were analyzed by the polymerase chain reaction(PCR) .Laboratory blood tests and body mass index (BMI) were analyzed in 103 biopsy-proved NASH patients and in 150 healthy volunteers. After providing informed consent, all NASH patients underwent abdominal computed tomography(CT). Liver biopsy specimen of NASH were analyzed by a pathologist(H.E) and the diagnosis of NASH were based on brunt's criteria.Results:D3 allele of the GHR gene was significantly lower frequent in NASH patients than in healthy volunteers(p<0.01),and carriers of d3/- genotype were significantly lower frequent in NASH patients than in healthy volunteers(p<0.01). D3/-NASH patients had a lower level of LDL-cholesterol compared to the fl/fl NASH patients did.Conclusion: D3 allele of GHR could be a candidate molecule that decrease the susceptibility to NASH, because d3 allel frequency in NASH patients was significantly lower compared with that in healthy volunteers and d3/- NASH patients had a lower level of LDL-cholesterol compared to the fl/fl NASH patients did.