Study On The Protection Of Bifidobacterium By Microencapsulation And Tablet Coating

The extension of preservative time of Bifldobacterium lyophilized powder was studied by air-suspension microencapsulation and Tablet coating. The main research contents and results were as follows:The small microencapsulating apparatus was self-signed according to air-suspension (Wurster process) principle, and parameters of the key parts of the apparatus were gotten. Bifldobacterium powder was micro-encapsulated by air-suspension process with the material A. which wasintestinal-lysis phragmoid polymer. After repeated experiments, the bestworking conditions were as follows:The composition of hydatid fluid: A(w/w) 5%; platicizer(PEG6000) 10% of A; solvent 95% ethanol;Working conditions: the total airflow(M3/h) 4.1-4.8; fluid-bed flow 3.0-3.5; the temperature of fluid-bed flow(C) 35-40, the airflow though air tube(MVh) 1.2-1.5; the amount of materiel disposal(g) 20-30; the amount of hydatid fluid(g) 80-120; the operation time (h) 1.5-2.0.The microcapsules were graininess which had even size, and disintegrated in artificial intestinal juice(pH6.8) with 45 min while they kept 2 hr intactly in artificial gastric juice(pH 1.2).The accelerated tests indicated that Bifldobacterium microcapsules showed more excellent keeping quality than Bifldobacterium powder which had no protection. After a three-month test under 37/RH60-65% (which amount to one-year store under normal temperature), the survival ratio of living cells in microcapsules was 20.3%. The microcapsules were suggested store in arid environment avoiding sunlight.The coated tablets in which Bifldobacterium powder was added were acid fastness and intestinal-lysis, but they were not as good as microcapsules.