Study Of Microencapsulating Peripheral Nerve's Tissue And Cells Transplantation In Stimulating Peripheral Nerve Regeneration

The regeneration and repair of peripheral nerve injury is an important question for study or discussion of neuroscience, trauma surgery and microsurgery. One of the hottest topic is to enhance the nerve regeneration's microenvironment and the effective method . Nerve growth factor(NGF) is a kind of the neurotrophin factors. It's produced by SC in peripheral nerve. It's well known that NGF can promote the regeneration of peripheral nerve and has been extensively used in the studying of nerve injury since it has been discovered. Recently, some experiments manifested that ground SC and NGF can be used in the surrounding of injuring of nerve or injecting into subepineurium and regeneration chamber to promote regeneration. But it has much limit to use it in clinic. As a kind of protein, NGF can be quickly degeneration in body. So it has to use it frequently and need special equipment. It's not very convenient. We used the Schwann cells' ability to promote regeneration of PN and designed this experiment. It concluded two parts: 1 we used the technology of microencapsulating tissue and cells and microencapsulated the SD rats' sciatic nerve tissue and cells. Then we used MTT test and ELISA method to study the activity of the microencapsulated tissue and cells; 2 Establishing the model of rat's sciatic injury, we studied the effects after the microencapsulation was transplanted by means of methylamine blue stain, immunohistochemistry electronic microscope, feedtrail test, electrophysiology in order to find the new clinic method of treatment of the injury of the PN.Result: 1 after the microencapsulating, the tissue of PN can survive at least 6 weeks. The OD value of Nol, 2, 3 weeks rose and then descended. It's said that the tissue of PN had proliferated in the microencapsulation. ELISA test showed that the tissue of PN in the microencapsulation had the ability of secretion of NGF. 2 From the comparison between the group A, in which transplanted the microencapsulation containing the PN tissue, and the group B, in which transplanted the microencapsulation without the PN tissue, and the group C, in which added the same volume of Saline. The amount of regenerating axons was larger in group A than in group B, and the myelin sheath was thicker as well. The regenerating nerve fibers of group A were parallel, and were arranged orderly, on the contrary, those of group B were arranged disorderly. The recovery time of the regenerating axons'ultrastructure last shorter in group A than in group B( P<0.05).Conclusion: 1 PN tissue and cells can survive after microencapsulated at least 6 weeks in vitro condition; 2 the microencapsulated PN tissue and cells can secretion some quantity of NGF but it was descended; 3 the transplantation of this microencapsulation in the location of PN injury can promote the regeneration of PN.