Study Of VEGF-A, VEGF-C And MVD Expression In Colorectal Adenoma And Carcinoma

Objective: To investigate relations of VEGF-A, VEGF-C and MVD expression colorectal adenoma and carcinoma Methods: The expression of VEGF-A, VEGF-C and MVD were studied by using S-P immunohistochemistry in 12 cases of normal large intestinal mucosa ,18 cases of colorectal adenoma, 81 cases of colorectal primary adenocarcinoma, 19 cases of lymph node metastase and 18 cases of liver metastase . Result: There was no positive expression of VEGF-A and VEGF-C in the 12 cases of normal large intestinal mucosa, whereas there was low positive expression rates in 18 cases of colorectal adenoma. The differences between normal large intestinal mucosa and colorectal adenoma were not significant(PVEGF-A=0.503,PVEGF-C=0.130). The positive expression rates of VEGF-A and VEGF-C in colorectal primary adenocarcinoma were higher than they were in normal large intestinal mucosa and colorectal adenoma, the differences were significant (P=0.000,P=0.008). There was no significant difference in the high expression of VEGF-A between the 19 primary adenocarcinoma cases and their corresponding lymph node metastases(P=0.319), whereas there was significant difference in the high expression of VEGF-C(P=0.020).Interestingly, the results showed that the positive expression rates of VEGF-A andVEGF-C in 18 cases colorectal primary adenocarcinoma and their corresponding liver metastases were more than 50% ,without any significance (PVEGF-A =1.000, PVEGF-C=0.735). The expressions of VEGF-A and VEGF-C were related to Dukes stage. MVD was related to Dukes stage, carcinoma position, pathology type, differentiation and infiltrate depth. Conclusions: 1.The results suggested that VEGF-A and VEGF-C accelerated neovascularization strongly and played an active role on the occurence and development of colorectal carcinoma. Lymph node metastasis was related clearly to the VEGF-C express in colorectal carcinoma. The detections of VEGF-C and MVD may help predict clinical prognosis. 2. The results also showed that colorectal lymph node metastase obtained more strongly malignant invasion because of the high express of VEGF-C .