Primary Study On The Correlation Between CT Appearances And Vascular Endothelial Growth Factor, Proliferating Cell Nuclear Antigen Expression In Renal Cell Carcinoma

Objective: To study the correlation between CT appearances and the expression of vascular endothelial growth factor, proliferating cell nuclear antigen in renal cell carcinoma.Methods: The incidence in expression of VEGF and PCNA gene was determined by immunohistochemical techniques with specific mono clonal antibodies, and the grade of cell differentiation was studied in 40 paraffin embedded specimens of RCC respectively. All 40 patients with RCC randomly selected were examined and diagnosed by CT (plain scan and contrast scan), nephrectomy and pathology from 1995 to 2002, and 10 normal renal tissues were picked out.Results: (1) The positive expression rate of VEGF ,PCNA in renal cell carcinoma was significantly higher than that in normal renal tissues(p<0.05); ( 2 ) The positive expression of VEGF was significantly correlated with tumor size ( diameter > 5.0cm),intratumor necrosis, liquefaction,cystic degeneration, tumor enhancement(CT value variation ≥20Hu ),poorly defined margin,perirenal fat infiltration, lymph nodes metastases,invasion of renal vein or inferior vena cava,invasion of adjacent organs or distant metastases on CT scan(p<0.05);(3) The positive expression of PCNA was significantly correlated with tumor size ( diameter>5.0cm ), tumor enhancement(CT value variation ≥ 20Hu ), poorly defined margin and perirenal fat infiltration on CT scan (p<0.05);However, there was no significant difference in PCNAexpression if tumors had intratumor necrosis,liquefaction,cystic degeneration,lymph nodes metastases,invasion of renal vein or inferior vena cava,invasion of adjacent organs or distant metastases (p>0.05);(4) As nuclear grade increased,RCCs were more likely to be positive VEGF and PCNA expression. (p<0.05).Conclusions: The detection of VEGF and PCNA gene expression in RCC combined with CT features was useful in early and exactly diagnosing of RCC and more objectively evaluating the biological behavior ,malignance and prognosis of RCC.