The Study On Adsorptive Effect Of Activated Carbon To Small Molecular Uremic Toxin And The Influence Factors

Objective:To elucidate the adsorptive effect of activated charcoal on uremic toxin, and the influence factors to the adsorptive effect of activated charcoal on uremicMethod:To make uremic toxic solution: urea, creatinine and uric acid were dissolved with distilled water. Then the uremic toxic solution was divided into different groups:(l) 20mg Activated charcoal was added to 250 u 1 uremic toxic solution, and every 10 minutes a reaction in a group was ended, the longest reactive time was 120minutes.(2) lOmg Activated charcoal was added to 150y 1 CRF blood serum, and there was 4 groups according to different reactive time.(3) Ten mg or twenty mg Activated charcoal was added to two different CRF blood serum groups of 250 u 1, seperately.(4) To establish interfered groups, the uremic toxic solution groups were added with activated charcoal in the presence of bile, glucose, amino acid, Ringer's, NaOH or HC1 separately. At the same time, We set a positive control: the uremic toxic solution was added with activated charcoal in the absense of the interfering factors; and a negative control: the uremic toxic solution was only added with distilled water as.Result:1 .Concentrations of toxins in uremic toxic solution began to decrease at 5 minutes after activated charcoa was added. The concentration of urea was lowest at 30 minute, but increased after 50 minutes; while creatinine and uric acid concentrations decreased to the lowest, between 10 to 30 minutes after activated charcoa was added, then they didn't fluctuate much after that.2. Concentrations of toxins in different interfered groups were not the same: (1) Uric Acid: there was no significant difference between the Interfered groups and positive control (p>0.05); (2) Urea nitrogen: there was no significant difference between the Ringer's group and positive control (p>0.05), but they were significantlylower than the other interfered groups and negative control (p>0.05). (3) Creatinine: among groups of pH2.0, glucose, pH7.9, Bile and amino acid, they were significantly different from those of the two control groups and other interfered groups, and they were not the same with each other (p<0.05 ). Conclusion:1. Activated charcoa can adsorb uremic toxins effectively, but the efficacity declines as the time goes on.2. Bile, glucose, amino acid, pH value of environment interfere activate charcoa adsorbing uremic toxins with different result.