Protective Effect Of Inosine Against Intrastriatal Kianic Acid Injection Induced Striatal Lesion In The Rat

Neuronal death occurred in various kinds of the CNS injuries and plays a critical role in the process of the CNS degenerative diseases. Preservation of neuronal viability is a major focus in clinical practice. Neuronal death, either necrosis or apoptosis, involves different mechanisms. Thus, different measures are needed to protect neurons under different situations. A large number of studies have been conducted aiming to ameliorate neuronal death. Inosine, one of the catabolic products of purine nucleotide, has long been clinically used to supplementally treat a variety of diseases due to its involvement in energy synthesis. Recently, it has been reported that inosine also plays an importanttrophic role in the CNS. In present study, loss of NADPH diaphorase (NADPH-d) neurons after KA intrastriatal injection served as a model to examine protective effect of inosine.In this study, the rats were administrated with kainic acid by intrastriatal injection. Then the lesion was evaluated by nissl stain and NADPH-d histochemistry. The size of the lesion area and the survival NADPH-d positive neurons were used to quantify the neuroprotective effect. At first, we had investigated the changes resulting from the different survival time after surgery and different dose of KA intrastriatal injection. The result suggests that the data is easily to be quantified when the animals were allowed to survive 3 days after low dose KA administrated. Then inosine and saline were both i.p. injected to show there effect on the KA induced striatal lesion.Our study reveals that inosine can decrease the size of lesion area and increase the survival NADPH-d positive neurons after intrastriatal injection of KA.