Experimental Studies Of 5-Fu Gelatin Microsphere (FGM) On Embolization Effects For External Carotid Artery And Branches In Dogs

Experimental studies of 5-Fu gelatin microsphere(FGM) on embolization effects for external carotid artery and branches in dogsPostgraduate Ma Qinqin Tutor Feng XinghuaTranscatheter Arterial Embolization(TCAE) is a kind of technique of using superselective intubation means to send embolic agents into local arteries in order to decrease or block local blood supply to cure the lesion with minimal trauma. In recent years, anticancer drugs were combined to several kind of vector (drug-containing microsphere) to treat carcinoma, this is called as chemoembolization. It is high effect, minimal trauma,and light side effect . with the development of TACE and the study on agent of chemoembolization, now TCAE has become a main part of the tumors' complex therapy and been put more and more attentions on.The selection of embolic agents plays a key role in interventional therapy. In our study, we adopted 5-Fluorouracil Gelatin Microspheres made by our hospital, diameter : 100-200um, drug content rate: 12%, encapsulation rate: 85%.To evaluate the safety and embolization effects of 5-Fu gelatin microsphere(FGM) in external carotid artery and branches ,under the supervision of X-ray, FGM was perfused into the external maxillary artery of four dogs and lingual artery of another four dogs, lOOmg for each.-4-Angiography was made after Smiru?7d?15d?30d of the treatment respectively, and blood flow velocity and volume were tested at different time after embolization, all the data were analyzed statistically. The sections of embolized tissue at different time were examined microscopically. We found that, the ends of embolized arteries could not be detected by angiography made immediately after embolization. The blood flow velocity and volume of the embolized artery decreased distinctly without the formation of collateral circulation, while the blood flow volume of adjoining arteries increased compensatively. FGM were found to closely stuff the cavity of the artery from the histological examination. In ends of embolized arteries ,we had found a fow FGM, around which there were a lot of red bloods accumulating and thrombosis, on the other hand, we didn't find inflammatory cells in the wall of arteries or FGM in veins, nor did find any necrosis of lingua or facial tissues. After half a month ,the FGM could be found abnormity and degraded in the tissues section.Studies were conducted in dog to evaluate the pharmacokinetics of 5-Fu gelatin microsphere(FGM) by the analysis of different employment methods of 5-Fu, eight dogs were divided into two group at random , FGM was perfused into the external carotid artery of dog by selective artery embolization under the supervision of X-ray, 5-Fu solution was infused into the external carotid artery and femoral vein as control, blood samples were tested at different time and analyzed statistically. We found that, a transient higher blood drug concentration(BDC) was produced by artery perfusion. Small dosage of FGM by method of artery embolization could produce a high BDC level for a longer time in local region, Ti/2 and MRT of embolization group are near 10 folds and 30 folds longer than that of the group of artey perfusion, there also were a discriminated difference in AUG.-5-The study show that external carotid artery branches embolization with FGM,which significantly reduced the circulating drug level and employment dosage, could increase therapeutic effects and reduce toxicity, could achieve the purpose of targeted tumor therapy. The study proved that, the FGM is a kind of safe biocompatible temporary agent, could embolized artery completely and be considered to treat the diseases clinically.