Studeis On The Effect Of HLJDC On Preventing And Curing Cerebral Ischemia

In this paper, the effect of preventing and curing cerebral ischemia and its mechanisms of HLJDC were investigated. The acute toxicity and the chronic toxicity were also investigated.The results of the cerebral ischemia experiments showedthat the HLJDC (0.70,1.40,2.80g/kg) inhibited the formationof carotid thrombosis induced by cotton thread in therabbits. The HLJDC ( 0.68, 1.35, 2.7g/kg ) apparently reduced neurologic deficits score and infarct size produced by MCAO in rats. The HLJDC ( 1, 2, 4 g/kg ) also reduced the stroke index score after cerebral ischemia in mice. Meanwhile, The HLJDC (2, 4, 8g/kg ) prolongedboth the gasping time of isolated heads of miceby decapitation and the survival time of mice subjectedto bilateral carotid ligation. It was suggested that theHLJDC protected the brain against ischemia. Moreover,the HLJDC (1, 2, 4g/kg) inhibited the pulmonary embolism. Thrombin time (TT), prothrombin time (PT), KPTT inthe rabbits (0.70,1.40,2.80g/kg) and clot time in mice (2,4, 8g/kg) were prolonged significantly (p<0.05 or p<0.01, vs control group), all results above suggested that the HLJDC oppressed blood thromboxane system. Besides, the HLJDC( 0.70,1. 40,2.80g/kg ) also improved blood rheology in ratsand inhibited platlet aggregation induced by ADP in rabbits. The HLJDC ( 0.20, 0.80g/kg ) increased cerebral blood flow (CBF) in anesthesized dog significantly butdid not effect the oxygen consumption index ofmyocardium. The result of acute toxicity experiment of the HLJDC showed that the toxicity of capsule wasrather safe, at the maximum dose of the HLJDC of 24g/kg(crude drug 106.56g/kg , single dose) , 48g/kg (crude drug213.12g/kg, daily, 54.6 times higher than clinical equivalent dose) , no toxicity reaction to HLJDC was observed. The chronic toxicity performed by oraladministration showed that at the dose of 5.4, 54, 108g/kgfor 3 months, no obvious toxicity was found. Resultsobtained from recovery period showed that the HLJDC had no accumulating and delaying toxicity .All experimental results above indicated that the preventing and curing mechanism of the HLJDC oncerebral ischemia were : (1) it inhibited platlet aggregation; (2) it oppressed the activation of thromboxane system; (3) it improved the blood rheology; (4) itincreased cerebral blood flow; (5) it could improve micro-circulation...