The Expression Of Fos Protein And NOS In Brain Following Liver Ischemia-Reperfusion Injury In Rats

Objective :To observe the changes of nitric oxide synthase (NOS) and fos protein in injuried brain following liver ischeraia-reperfusion injury and evaluate the contribution of NOS and c-fos protein to brain tissue effect.Methods:A model of liver ischemia-reperfusion injury was established by imitation . The contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were examined individually by immunohistochemical technique following shamoperation(groupl), ischemiaSOmin without reperfusion(group2), reperfusionfollowing schemia30min(group3). 1 hour reperfusion following ischemiaSOmin (group4). 2 hour reperfusion following ischemiaSOmin (groupS) and 4 hour reperfusion following ischemiaSOmin (groupG).ResultsrCompared with group!,group2 and groupS, the contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were little statistic significance, (P>0. 05). There was obviously statistic significance between group4 and former groups, (P<0.05). Theincrease exists remarkably about the contents of NOS and c-fos protein in hypothalamus compared with former groups in themselves(P<0. 01). There also was higher level in group5 than the former groups(P<0.05), while no changes happen between groupS and group6(P>0. 05).Conclusion:The data of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions vary depending on different time of liver ischemia-reperfusion specially, after reperfusion. NOS and c-fos protein are involved in brain tissue injury following reperfusion of ischemia liver.