Analysis Of Gene Expression Patterns In Cervical Squamous Cell Carcinoma Of Uygur By CDNA Micro Array

Objective To investigate the differentially expressed gene in cervical squamous cell carcinoma of uygur and normal cervical tissue using gene chip. Methods The PCR products of 2048 genes were spotted onto a chemical-material-coated-glass plates in array. The DNAs were fixed onto the glass plate after series of treatments. The total RNAs were isolated from the tissues, and were purified to mRNAs by Oligotex. Both mRNAs from the cervical squamous cell carcinoma and the normal tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues. Results By applying this gene chip we identified 64 differentially expressed genes in all 3 clinical cervical cancer samples, and we further identified 53 upregulated and 11 downregulated genes for functional analysis. Conclusion The analysis of gene expression pattern of tumor based on gene chip can realize high-throughput screening of the genes associated with the cervical cancer. Further analysis of the obtained genes will help to understand the molecular mechanism of tumor carcinoma.