| The asphyxia of newborn is one of the most important causes which leads to neonates death and permanent disability. Recently, many studies have demonstrated that lots of cytokines are released and act important roles in asphyxia neonates. Previous reports focus on the changes of cytokines in vein serum, cerebrospinal fluid ( CSF) or bronchus alveolus fluid ( BALF) after asphyxia and vein serum or am-niotic fluid of mothers, which obtain some developments. However, these examinations would take some injuries to mothers and neonates. There are few reports about the study on the changes of cytokines in the cord blood of asphyxia neonates abroad and no reports domestic. Our objective is to assess (1) The influence of asphyxia on the levels of interleukin - 6 (IL - 6 ) , interleukin - 8 (IL - 8) and tumor necrosis factor - alpha ( TNF - a) in cord blood of full - term neonates. ( 2 ) The changes of IL - 6, IL - 8 and TNF - ain cord blood between mild and severe asphyxia. (3 ) Weather the mode of delivery influent the level of cytokines.Material and MethodsBetween March 2002 and December 2002,143 neonates who were born in our hospital were initially enrolled in this study. Among them, 80 were selected in the study according to the following criterions : ( 1) gestation more than or equal to 37 weeks; or less than or equal to 42 weeks. (2) Single pregnant infant . (3) Without evidence of major congenital malformation (4) the neonates whose mother been seriously ill were excluded.Neonates were classified into two groups according to the mode of delivery ( vaginal delivery group 34 and caesarean section group 46 ). Two groups according to the presence (40) and absence (40) asphyxia .Three groups according to the mild(30) or severe(10) or absent (40) of asphyxia.The blood samples were obtained from cord veins immediately after the born of infant. The blood was centrifuged 10 minutes and serum was stored at -20癈 until assayed.The enzyme - linked immunosorbent assay ( ELJSA) method was used with commercially available kits . ( Jingmei Corporation , Beijing , China)The data were analyzed with SPSS 11.0 For Windows and described as x ?s. The significance of difference was determined by two - tailed t test and ^ determination.Result1. IL -6, IL -8 and TNF - ain the cord blood have no differ-ence between vaginal delivery and caesarean section (425.48 ±172. 23 vs 402.01 ± 169.10 P >0.05; 514.12 ±369.27 vs 417.60 ±254. 63 p>0.05;23.94±24.48 vs 28. 29 ±20. 20 p>0.05) . In addition , cord blood levels of cytokines in asphyxia neonates also have no statistically difference no matter what the mode of delivery ( p > 0.05).2. In contrast , we found that levels of IL - 6 and IL - 8 increased while TNF - adecreased significantly after asphyxia compared with those of no asphyxia (488.95 ± 110. 80 vs 342.01 ± 188.03 p < 0.001 ;723.46 ± 237. 28 vs 222. 73 ± 178. 86 p < 0. 001; 12. 00 ± 6. 04 vs 39.57 ±24.89 p <0.001).3. Moreover , severe asphyxia led to a significant increase in IL -6 and IL -8 levels compared with mild asphyxia (607. 73 ±95. 69vs449.36± 84.75 p<0.001; 958.52 ±223.52 vs 645.11 ±186.57 p<0.001).DiscussionInflammatory cytokines are the cytokines which are characterized as inflammatory medium activities . They are kinds of with similar structure small molecules, including IL - 6 , IL - 8 and TNF - a et al. Biologically active cytokines are released from many kinds of placenta cells . They act double roles as protection and injury. Our studies showed that IL - 6, IL - 8 and TNF - awere not affected by the mode of delivery (p >0. 05) , which consistent with the conclusion of Mickael et al. We also found that levels of IL - 6 and IL - 8 increased while TNF - adecreased significantly after asphyxia ( p < 0. 001). In addition, severe asphyxia led to a significant increase in IL - 6 and IL- 8 levels compared with mild asphyxia . The concomitant decrease in TNF - a and increase in IL - 6 and IL - 8 could be attributed to an inhibitory effect of IL -...
|
PDF Full Text Request