| Ceramic has been applied on prosthodontic clinic widely because of its biocompatibility, wear resistance and good esthetics. It is a im-portant problem to dentist and patients that ceramic have weak strength. Infiltrated Ceramics have high strength. Before a new mate-rials will been applied the test on its biologic compatibility is needed. There are many vitro cell culture techniques that evaluate the compati-bility of new materials. Our study used methabezthiazuron ( MTT) method and phase - contrast microscope to evaluate the compatibility of new infiltrated ceramics with machinable properties.SubjectiveStudy the compatibility of new infiltrated ceramics with machin-able properties and offer the basis of its clinical apply.Material and methodMaterials: After the disks were made, they were socked in dichlo-roethane for l0min with shaking. Ultraviolet ray light on their each surface for 24h. Extraction medium was added to each sample at 0.55ml/cm ration. Extraction was performed in an atmosphere of 5% co2, 95% air at 37~???C for72h.Method; Contrast microscope: After 24h, 36h, 48h, 72h of growth, observance were made using a contrast microscope and take pictures for every period. MTT assay: Tests were conducted after 24h,36h,48h,72h of growth of L -929 cells on the various extracts in 96 -well plates. 2mg/ml,50ul MTT solution was added to each well. After 3h incubation, the supernatant was removed and the formazan crystals dissolved by adding 150ul DMSO to each well. After shaking for l0min,absorption was measured at 490nm.ResultsContrast microscope.- Cell morphologies were the Same except cells in positive control. MTT assay: An analysis of variance showed significant differences between positive control and other groups.DiscussionInfiltrated ceramics changes the present of oral material. This ce-ramics have high flexural strength. Infiltrated ceramics with machin-able properties will bring entrance for prosthodomtic. In order to gel-ling alumina fabric, the dry alumina powder is pressed and the alumi-na powder is made in lab personally. After pre - sintering of alumina fabric in low temperature, the mica micro - crystalline glass is infiltra-ted into the poreus alumina matrix. The intensity of infiltrated ceramics increases into about 3 times.Morpholgy: The morphology study showed that there were no im-portant differences between infiltrated ceramics with machinable pror-erties with porcelain. MTT assay didnt reveal a clear ditterence be-tween the number of cells present on the porcelain as compared to the porcelain with the infiltrated ceramics with machinable prorerties . In-deed, the number of cells was significantly lower in positive control than in other groups.Brief SummaryThis experiment used methabzthiazuron(MTT) method and phase - contrast microscope to evaluate the compatibility of infiltrated ce-ramics with machinable properties. This new material induces no sig-nificant difference with porcelain and a significantly lower cytotoxity than the pb positive control.ConclusionThe following conclusions were drawn from the shear testing re-sults obtained in this study:KThe cytotoxicity of new infiltrated ceramics with machinable properties were grade 0.2 This study has revealed a relative bio compatibility of new in-filtrated ceramics with machinable properties when compared to Japan Porcelain... |
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