Study On The Apoptosis Of Prostate Cancer Cell Pc-3 Induced By Huazhengjiaonang

Objective: To investigate induction of apoptosis in huazhengjiaonang~treated pc-3 cell line. Methods: MTT assay was used to detect the half~inhibition concentration (ICso) of huazhengjiaonang on pc-3 cell line, then the huazhengjiaonang with appropriate concentration was chosen to treat pc-3 cells for a certain time. Morphological changes of apoptotic cells were investigated. The pc-3 cells apoptosis induced by huazhengjiaonang was investigated by using a series of quantitative and qualitative methods. DNA fragmentation was analyzed by agarose gel electrophoresis. Additionally, the number of apoptotic cells and the DNA content were quantitatively assessed by flow cytometry (FCM). Results: The pc-3 cells treated by huazhengjiaonang revealed many characteristic morphological features consistent with apoptosis, such as the cell growth inhibition, the proliferation speed decrease, the cells rounded-up and shrunken. The transmission electron microscopic analysis of the ultrastructural changes occurring in pc-3 cells treated by huazhengjiaonang revealed some morphological changes which included altered cytosolic density, pronounced vacuole formation, chromatin condensation and nuclear fragmentation at the margin of nuclear envelope, the dilation of cytoplasmic organelles such as mitochondria and rough endoplasmic reticulum, and the apoptotic body formation. At the same time, the pc-3 cells were induced the specific biochemical changes consistent with apoptosis by huazhengjiaonang. For example, the agarose gel electrophoresis of extracted genomic DNA from huazhengjiaonang treated cells showed the fragmentation of genomic DNA into laddering patterns. The DNA content analysis of FCM showed pre-G1 cells. In the experiment range, the apoptosis inducing effect of huazhengjiaonang was dose" dependent. Conclusion: Huazhengjiaonang can kill pc-3 cell by inducing the cells' apoptosis. The apoptosis effect is dose-dependerit.