Effect Of PARP On Apoptosis And Proliferation In Androgen Independent Prostate Cancer PC3and In Androgen Dependent Prostate Cancer LNCaP Cell Line

Objective To investigate the biological role of5-AIQ-induced PARP inhibitionon apoptosis and proliferation in Androgen independent Prostate Cancer PC3andin Androgen Dependent Prostate Cancer LNCaP cell lineMethods without treatment of5-AIQ,the difference expression of PARP innormal prostate epithelial cell RWPE-1and prostate Cancer cell PC3, LNCAP wasdetected by Western blot analysis. After treatment with different concentraton of5-AIQ, the expression of PARP in PC3and LNCAP was detected by Western blotanalysis. The proliferation of PC3cells was detected by MTS assay, and apoptosis ofPC3cells was detected by flow cytometry analysis.Results The expression of PARP in PC3and LNCaP cell line was significantlysuppressed to normal prostate epithelial cell RWPE-1. The expression of PARP inLNCaP and PC3cell line was remarkablely decreased in5-AIQ treatment group.The proliferation of LNCaP and PC3cells was significantly inhibited after5-AIQtreatment compared with blank group (without treatment)(p<0.05), with thedose-time-effect relationships. Flow cytometry analysis showed that the number ofcells in early, late and total phase apoptosis induced by different doses (500μmol/L or1000μmol/L) of5-AIQ were significantly higher than those in blank group (P<0.05).Conclusion The expression of PARP in LNCaP and PC3cell line was suppressed byPARP inhibitor5-AIQ, which can both inhibit proliferation and induce apoptosis of theLNCaP and PC3cell line, PARP was expected to become a new therapeutic target forprostate cancer in the future.