| Objectives: Hepatopathy ,which impairs the survival rate and the quality of lives seriously, is a kind of common disease in China. Liver fibrosis is the commonly pathological basis and inevitable stage when some etiological factors(such as: hepatitis virus, alcohol, blood flukes)are caught, and chronic hepatopathy, which would develop into cirrhosis or liver cancer, is evoked by the immune mechanism. Therefore, the key point to block the vicious cycle is to prevent,cure and invert liver fibrosis. The pathological character of liver fibrosis is that too much extra cellular matrix(ECM) is deposited outside the liver cells because ECM is synthesized too much, and its degradation is inhibited. Hepatic stellate cells(HSC) are the major cells to synthesize collagen in the liver. HSC is non-active under common condition; Their ability to synthesize collagen is strengthened when HSC are activated. So the basic mechanism for the occurrence of liver fibrosis is the activation and proliferation of HSC. The major way to prevent and invert liver fibrosis is to inhibit the activationand proliferation of HSC, to evoke the HSC activated to apoptosis, and to accelerate the degradation of collagen. Now, no ideal way to treat the chronic hepatopathy has been found in western medicine; At the same time, an promising effect has been noticed when the Chinese medicine was applied in the treatment of liver fibrosis. The action mechanism of Danshen and Yigankang, which had been proved effective clinically in treating liver fibrosis, and the effect of conversion products of Chinese herbs in vivo to the phenotype and function of HSC are to be explored by the way of cells and molecular. Colchicine was adopted as the control. The effects of Danshen,Yigankang and colchicines on the LP,NF-κB and the activation and proliferation of HSC were observed, and the mechanisms and effects of the 3 different drugs were compared. At the same time, the "improved seropharmocological method", which has some difference from the traditional one, was used in this investigation, and the difference between the two methods was analyzed.Methods: Pharmocological serum was extracted by the way of 2 different seropharmocological methods, then the serums were used in the cultivation of HSC, and the effects were examined.1 The production of pharmocological serum:①Clean,SD rats, 230-260g, 80, were randomized into 8 groups as follows(10 in each group):Group A: Normal rats Group A1: Danshen Group A2:Yigankang Group A3: colchicine Group A4: controlGroup B: Fibrosis rats Group B1: Danshen Group B2: Yigankang Group B3: colchicine Group B4: controlRats in Group B were modeled into liver fibrosis by the way of: 40％ CCl4, Subcutaneous injection, 2 times a week for 9 weeks. Rats in Group A were injected Saline subcutaneous by the same dosage and time as Group B.②2 rats were selected out randomly from Group A and 2 from Group B, then the pathological changes of liver were observed after 9 weeks.③The corresponding drugs were filled into the stomachs of rats : 2 times a day, for 5 days after 9 weeks. The dosage was 10 times that of an adult per day per kg. The rats had been fasted from the evening of the 4th day. The blood was extracted from the inferior vena cava at the time of 2 hours after the last time filling of drugs on the morning of the 5th day. The serum was separated by centrifugation under the condition of 4℃, 3000rpm, for 20 minutes, and was inactivated in 56℃, for 30 minutes, then was reserved in -70℃. ④The serum was sterilized by filtration, then was dispensed in the 8％RPMI-1640 culture mediun to make the 10％ drug serum-1640 culture medium.2 HSCs were cultivated in the drug serum and were detected. The 10％drug serum-1640 culture medium were changed into the cultivation system and HSC were observed after HSC stuck the wall in the 8％RPMI-1640 cultur...
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