| Objective: To study the influence of tonifying kidney recipes on osseintegration around Titanium Implants and investigate the mechanism of the effect of tonifying kidney recipes on osseointegration of the Titanium Implants by established animal model of osteoporosis rats.Methods: Fifty-four female SD rats (8-month-old) were randomly divided into three groups, 18 rats in each group, They included a sham-operated group (Sham group), an ovariectomized group (OVX group) and a tonifying kidney recipes therapy group (TKR group), After 3 months, the animal models of osteoporosis were established, pour titanium smooth columnar implants were placed in the proximal metaphyses of left tibiae of the rats. One day after the implant surgery, tonifying kidney recipes was given orally at the dose of 0.32g/100g/day in the TKR group and normal saline was given to the rats of the other two groups. At 1st,2nd,3rd months after surgery the rats were killed respectively and the implanted tibiae were got , the tissue samples were fixed with 10% Formaldehyde soaked and 8% formic acid was used to dissolve the calcium for 10 days. After rinsed 24 hours with water, the tissue samples were embedded in paraffin and were sectioned with a thickness of 5μm. The sectionswere stained with HE for histological examination and bone histomor- phometric parameters were measured to quantify the changes of new bone around titanium implants and to observe the changes of implant-bone interface.Results: 1.At the 3rd months after ovarictomy surgery, the weight of rats increased significantly in ovariectomized group compared with sham group(P<0.01), the weight of rats increased significantly in TKR group(P<0.01); while there were no significant changes of weight between the OVX group and the TKR group(P＞0.05). Meanwhile, the changes of bone mineral density (BMD) of the three groups were measured by densitometry. The BMD of the rats in Sham group increased significantly compared with OVX group (P<0.01);the BMD of the rats in sham group increased significantly compared with TKR group, but there were no significant changes of the BMD of the rats between OVX group and TKR group (P＞0.05).The results showed that the animal models of osteoporosis rats were established by ovarictomy.2.The results observed by light microscopy showed as follows:At the 1st month after implant surgery, in the sham group, the new bone in cortical bone area was mainly formed by woven bone which was in direct contact with implants in cancellous bone area, the combinded bone lamella was thicker, the density of trabecular bone was greater; the OVX group, the combinded bone lamella was thin and was not contacted with implants somewhere, there were a lots ofosteoblasts(OB) and osteoclasts(CB). Haversion system in cortical bone area was immature; the TKR group, the interface bone lamella were thicker than OVX group, the contact of new bone around implant and the osteoid perimeter were greater than OVX- group, trabeculars were concentrated, there were a lots of osteoblast with larger cell nucleolus, osteoclast were sparsity. At the 2nd month after implant surgery, the Sham group combinded bone lamella was thicker and the surface of it was smooth, the density of trabecular bone were greater than that of 1st months after implant surgery; the OVX group, combinded bone lamella was thin, a lots of osteoblast and osteoclast could been saw; the TKR group, combinded bone lamella was thicker, osteoblasts were massive, and osteoclast were sparsity.At the 3rd month after implant surgery, The woven bone was substituted by lamellalike new bone in cortical bone area in the three group, the density of trabecular bone and contact of new bone around implants in Sham group and TKR group were greater compared with OVX group. 3.The results of bone histomorphometry were as follows:At the 1st month after implant surgery, Trabercular Area (Tb·Ar%), Trabercular Width (Tb·Wi), Combinded Bone Lamella Width (CBLW) in Sham group were significantly increased compared with O...
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