| Objective:The purpose of this experiment were (1)to determine nontoxic and effective dosage concentration of Sulperazon solution for intravitreal injection.(2)to correlate clinical signs with progression of intraocular degeneration and B scan ultrasonography.(3)to evaluate the efficacy of intravitreal Sulperazon in the treatment of experimentally induced Pseudomonal endophthalmitis.Methods:1 The study of the sulperazon toxicity to retinaFifteen healthy rabbits were divided into five groups.There were six eyes in each group. The dosage of each group were as such:0mg/0.1ml, 5mg/0.1ml, 10mg/0.1ml, 20mg/0.1ml, 40mg/0.1ml.The animals were examined clinically at 1,2,4,8,12,24hours and every day after injection. Compared the results of ERG of 1,3,7,14days after injection with it before injection. The animals were killed and enucleated at 2 weeks after injection and prepared for histopathologic observation. The a-wave ,b-wave amplitude of ERG were analyzed statistically using analysis of variance.2 National History Pilot StudySix rabbits were used in this section of our experiment.About1000 Pseudomonas anruginosa were injected into the midvitreous of each experimental eye. The animals were killed and enucleated at 12 ,16,20,24,30 and 44 hours after inoculation and prepared for histopathologic observation.3 Efficacy of Sulperazon3.1 Eighteen rabbits divided at random into 3 groups after inoculated Pseudomonas anruginosa 6 hours. 6 rabbits received 10mg/0.1ml Sulperazon, 6 rabbits received 2.25mg/0.1ml ceftazidime, 6 rabbits received 0.1ml normal saline. The animals were killed and enucleated at 24hours after inoculation and prepared for histopathologic observation.3.2 Twelf rabbits inoculated Pseudomonas anruginosa and divided at random into 2 groups. 6 rabbits received 10mg/0.1ml Sulperazon after 6 hours, 6 rabbits received 10mg/0.1ml Sulperazon after 20 hours, The animals were examined clinically at 1,2,3and 7days using slitlamp biomicroscopy, indirect ophthalmoscopy and B-scan. The animals were examined with ERG before injection and 7days after injection.3.3 0.1 ml vitreal samples were obtained from 2 eyes of each group for culture before the animals were killed.Results:1 The study of the sulperazon toxicity to retina1.1 Clinical examation:There were no significant difference in these groups.1.2 Flash-ERG:(1) The waves of the first and the third daydeclined and were significant difference with those of normal(P<0.05). (2) There were no significant difference between 5mg group,10mg group and the control group with the normal of the first week and the second week(P>0.05). There were significant difference between 20mg group and 40mg group with the normal(P<0.05).1.3 Light-microscopy : There were no significant abnormality were seen in 5mg group,10mg group and the control group of the retina.The inter and outer photoreceptor segment of the retina of 20mg group were mild disorganization. The inter and outer photoreceptor segment of 40mg group became attenuate or even loss, all the structure were marked disorganization.1.4 Transmitting electric microscopy : There were no significant abnormality were seen in 5mg group,10mg group and the control group in the retina. There were significant abnormality were seen in 20mg group and 40mg group .2 National History Pilot Study2.1 Clinical examation: The first sign of inflammation appeared between six and eight hours.The infection reached a peak between 24 and 30 hours. 2.2 B-scan:There were local clouding at the site of the needle track after 6 hours of innoculation.18 hours later,the clouding diffused,local vitreaous detached,local retina exudative were seen.2.3 Histological:There was an exudate surrounding the iris at 12 hours. At 16 hours a collection of leukocytes was seen inthe exudate and the ciliary.The inflammation were severe with time. At 30 hours ,the vitreaous purulent formed.By 44 hours the vitreous was filled with leukocytes,all the structure had been distroyed. 3 Effica... |
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