Laboratory Diagnosis Of Fungal Endophthalmitis

Some patients suffered from fungal endophthamitis are endogenous infection, others are secondary to ocular operation or trauma. The prevalence rate of fungal endophthamitis is about 4%-11% in the culture-proven endophthalmitis. The rate is not high, but it' s prognosis is serious. If the patient can' t get early diagnosis and therapy, he will lose sight thoroughly. The very important factor to effect prognosis is early diagnosis and therapy. The traditional lab examination includes smear and culture of fungi. Somebody reports that smear is a convenience and fast method, but the positive rate is low; culture is "golden standard" to diagnose infective disease. The positive rate of culture is nearly to smear and the 2~ 14 days culture period limits it 's use in clinic. As a useful lab tool, PCR has high sensitivity. It is widely used in clinic.Candida albicans and Aspergillus fumigatus were researched. 30 rabbits were used as experimental animal. 50ul fungi solution was injected into right eye. 3 days before operation and 1st, 3rd, 7th, 14th and 30th daypostoperatively, aqueous humor and vitreous body were collected and used as samples in smear, culture and PCR respectively. Variance analysis was used to treat the data. The results are as follow:1. Aspergillus fumigatus did more harm to eyes than Candida albicans did. III stage of endophthalmitis as a standard, the period is 24 + 2. 1d in Candida albicans group and 14 + 1. 5d in Aspergillus fumigatus group.2. The positive rate of samples collected on 7th , 14th, 30th day has no difference (p >0. 005)and has difference with samples collected on 1st, 3rd respectively (P< 0.005) .3. The positive rate of vitreous body is higher than aqueous humor' s in smear (P< 0. 05) . Aqueous humor has the same positive rate as vitreous body in PCR and culture (p >0. 05).4. The positive rate of samples collected in Candida albicans group has no difference with that in Aspergillus fumigatus group in smear , culture and PCR(p >0.05).5. All samples were examined with smear, culture and PCR,...