| By investigating the composition principles in compound prescription of the Chinese traditional medicine by the pharmacokinetical method, this paper expounds the relationship among the dominant drug and others in the same prescription, according as the composition whether benefit the major active components of the dominant drug to be absorbed. Consequently, the investigation makes it possible to clarify the effect and status of the other drugs in the description, and lead to the further research.Objective:To establish a method to determine the quantities of Ephedrine(E) and Pseudo-ephedrine(PE) in the serum,and study the mutative rules of the pharmacokinetical parameters of the E and PE in different composition.Method and Design:1. A selective gas-chromatographic method with mass spectrometry (GC-MS) for the simultaneous confirmation and quantification of E and PE was developed in serum after derivatization with trifiuoroacetic anhydride (TFA). Orthogonal trial was used to select the optimal conditions such as alkali dose,NaCl dose and extractive solvent and derivation conditions.2.The mouse were divided into groups in random and orally perfused the medicine. On the points of 2.5,5,10,20,30,45,60,90,120,240,480,720min,picking off the eyeballs of the mouse got blood samples.3. The pharmacokinetical parameters of each composition werecalculated by the 3P87.4. The statistical analysis of the pharmacokinetical parameters such as Tmax,Cmax,AUC were proceeded by SPSS10.0.Results:1. The determination of the E and PE in mouse serum is better after being derived and using diphenylam as internal standard by GC-MS-SIM.2. E-Auc and E-Cmax of different compositions shows significant variance,.3. The PE pharmacokinetical parameters of different compatibility have not significant variance.4. The concentration-time curves of E and PE sometimes show double-kurtosis.Conclusion:Minister-drug,adjuvant-drug,etc. have some certain extent effect to the pharmacokinetical parameters of the dominant drug.
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