Study On Relationship Between Damp-Heat In Spleen Syndrome Of Chronic Hepatitis B And Serum Levels Of TNF-α,IL-6,SOD And MDA

Objectives: To study the serum levels of TNF-a, IL-6, SOD and MDA of Chronic Hepatitis B and evaluate the relationship between Damp-heat Syndrome and TNF- a ?IL-6, SOD and MDA. To explore the relationship betweens the material produced by Damp-heat and the damage to the liver and give service to the treatment based on the differential diagnosis of clinical. Methods: 124 patients of CHB were divided into two groups by syndrome differentiation of TCM, Damp-heat in Spleen Syndrome (DH) group (64 cases) and Deficiency of Spleen-Qi Syndrome (DQ) group (60 cases).DH group included 32 cases treated by routine treatment and 32 cases treated by integrated TCM and western medicine treatment. DQ group included 30 cases treated by routine treatment and 30 cases treated by integrated TCM and western medicine.30 cases healthy people were enrolled as normal control (NC) group. All subjects were hemospasiaed venomously under fasting condition. ELISA measured the markers including TNF-a and IL-6 before and after treatment, as well as SOD and MDA were measured by biochemistry. The marks of syndrome were recorded before and after treatment. Results:(1) The levels of TNF-a , IL-6 and MDA in serum of patients with CHB (including light degree, middle degree and heavy degree) were obviously higher than those of the NC group(p<0.01).The level of SOD in serum of patients with CHB was obviously lower than those of the NC group(p<0.01).There was close negative correlation between SOD and MDA, but close positive correlation between TNF-a and IL-6 .(2) There was close negative correlation between ALT and SOD, but close positive correlation between ALT and TNF-a, IL-6, MDA .The levels of TNF-a, IL-6, SOD and MDA were changed with the degree of thedamage of livcr.(3)Damp-heat in Spleen Syndrome was concerned with middle degree and heavy degree of CHB, as well as Deficiency of Spleen-Qi Syndrome was concerned with light degree of CHB.(4)The levels of TNF- a , IL-6 and MDA of CHB with Damp-heat in Spleen Syndrome and Deficiency of Spleen-Qi Syndrome were evidently higher than NC group (p<0.01).The level of SOD of them was evidently lower than NC group (pO.Ol).The change of DH group was clearer than that of DQ group (p<0.05).(5)There was close positive correlation between the mark of Damp-heat in Spleen syndrome and TNF- IL-6 and MDA ,but close negative correlation between the mark of Damp-heat in Spleen syndrome and SOD.(6)The levels of TNF- IL-6 and MDA of CHB after treatment were obviously lower than those before treatment (p<0.01) and the level of SOD was higher than before treatment greatly. The curative effect of integrated treatment in decreasing the levels of TNF- , IL-6 . MDA and increasing the level of SOD was super to those of routine treatment.(7)The curative effect of integrated treatment in improving symptom was super to that of routine treatment. Conclusions: There is relation among interaction of cytokine, free radicals and lipid peroxidatious in the occurring of CHB. The relation of material produced by Damp-heat and damage of liver was close. Damp-heat plays important role in the pathological change of CHB; The patient's condition of Damp-heat in Spleen Syndrome was more serious than that of Deficiency of Spleen-Qi Syndrome; The treatment integrated TCM and western medicine to CHB can improve the symptom obviously and lighten the damage of liver; The levels of TNF- , IL-6, SOD and MDA may reflect the part essence of Damp-heat Syndrome of TCM and they play role to the objectivity of syndrom...