| Objective To investigate nasal NK/T-cell lymphoma for its features of clinicopathology and immunophenotypes, the nature of the tumor cells , the relationships between NK/T-cell lymphoma and infection of Epstein-Barr virus. Methods The phenotype was determined by immunohistochemical with various antibodies. Genotypic study was examined by PCR-PAGE. The presence of EBV-encodeed small nuclear early region(EBER)RNA were analyzed by in situ hybridization.Results (1)The positive rate of CD56,polyclonalCD3,CD45RO and LMP-1 were 100%,82.6%, 67.4%, 19.6%, respectively.Tumor cells did not express antigens of B lymphocyte. (2)The positive rate of GrB was 91.3%. (3)EBER transcripts were indentified in 26 of 28 (92.9%) patients.(4)The rearrangements of T-cell receptor(TCR)-β gene was shown in 19 of 30(63.33%) patients. Conclusions These data suggest (1)CD56,polyclonalCD3 and CD45RO can be used as the most sensitive ,peculiar markers for identifying nasal NK/T-cell lymphoma. (2)Granzyme B may be used as an additional marker in the diagnosis of NK/T-cell lymphoma in conjunction with CD56.(3)EBV may play a role in the development of NK/T-cell lymphoma. However, the role of LMP-1 was limited.(4)The rearrangement of TCR-β indicated the presence of a monoclonal proliferation of the T-lineage.The nature of clonal did not change in the progression of the tumor.Nasal NK/T-cll lymphoma may be derived from bipotential common progenitor cellsof NK/T cell lineage. |
