A Meliorative Animal Model Of Experimental Allergic Encephalomyelitis

To induce the meliorative model of experimental allergic encephalomyelitis, we purified the myelin basic protein(MBP) from human brain and spinal cord, by means of defatting with methanol-chloroform, acid extraction and CM-52 chromatography. Then, SDS-PAGE, ELISA, and Western-blot had been used to identified the human MBP purified by our lab, which showed that the protein was a product with good purification and immunological activation, and can be combined with the human MBP antibody. Meanwhile, it can induce experimental allergic encephalomyelitis(EAE) in Wistar rat, which showed large demyelinated areas, lymphocytes recruitment, and neuron destroy in the brain and spinal cord like human with multiple sclerosis(MS). PBMCs and spleen cells from this EAE models proliferated highly to human MBP in vitro. In sera, the concentration of IFN Y and TNF a , secreted mostly by Thl cells, increased significantly when disease relapsed, while the concentration of IL-10, secreted mostly by Th2 cells, increased when disease remitted. Thus, we can concluded that the animal model of EAE induced by human MBP without BPV bear a resemblance to the features of MS, which was considered a better model than ever before for the study of multiple sclerosis. Finaly, we test the distribution of RANTES, one of the chemokines,by immunohistology and find that most of them distributed both in the endothelium cells of the blood vessels and infiltrated inflammation cells in the brain.