| The demyelinating disease of the central nervous system (CNS) is a disease which is often seen in youth. The etiology is now related to varies aspects, such as heredity, virus, immunity and enviorment etc. Although the definite etiology and pathogenesis are not very clear up to now, many studies show that the immunological factors play a key role in the demyelinating disease of CNS. It is assumed that the demyelinating disease of CNS resembles other antoimmune diseases in the illness process, and the autoantigens, nonspecific T cell activation and inflammatory reaction mediated by T cells are relevant to the disease. Myelin basic protein (MBP) is not only the important element of nervous myelin, but also the major antigen of the demyelinating disease of CNS. The Experimental allergic encephalomyelitis (EAE) induced by MBP with Freund's complete adjuvant (FCA) is a typical animal model to study the demyelinating disease of CNS, especially multiple sclerosis (MS) for its similarity with the human demyelinating disease of CNS in clinical manifestation, immunopathology andimmunochemistry. The method of animal splenic mononuclear cells and dendritic cells (DC) co-culture with MBP in vitro is a good way to study the onset and treatment of EAE. DC is the strongest antigen-presenting cell (APC) in body. It has been known that the cells have dual role in immunologic response and tolerance, and accommodate immune response in the body. According to the different kines of cytokine secreted by CD4+ cell, it can be divided into Th1 and Th2 cells. Th1 cells secreteIL-2.IFN- ,IFN- etc, conduct to cellular immunity; Th2 cells secrete IL-4, IL-5, IL-6, and IL-13 etc, conduct to humoral immunity or allergic diseases. These cytokines may interacted with each other and regulate the equilibrium of Thl/Th2 cells. Once the equilibrium were impaired, It would lead to the occurrence of many autoimmune diseases. DC1 induces Thl response, while DC2 induces Th2 response. Theoretically, DC1/DC2 can control the equilibrium of Th1/Th2 , therefore, the cellular immunity mediated by Thl cells or humoral immunity mediated by Th2 cells could be prevented.This experiment made use of in vitro cell culture method to separate the splenic mononuclear cells from BALB/c mice susceptible to EAE and co-culture them with MBP in different cultivation condition. The fluorescence Abs which were the notation Ags of anti-DCl and anti-DC2 were exerted to mark the cells. The proportion was detected by flow cytometer. The best cultivation condition (cytokine) which could make the DC precurcor differentiate more to DC2 was selected to stimulate the orientative differentiation from DC precursor to DC2, furthermore to modulate Th0 to Th2. This will establish an experimental foundation for the modulation of orientative differentiation from DC precursor to DC2 by medcine and furthermore down-regulating Thl function and up-regulating Th2 function in the treatment of demyelinating disease of CNS in order to promote the recovery of the disease and reduce the relapse of it.The main results of this experiment are as follows: (1)Co-culture of GM-CSF and IL-4, MBP could stimulate DC precursor to express extremely more CD11c. The mean fluorescence intensity of CDllc of study group was 856.93 12.45,while the control group was 827.05 17.38 , t=5.41,P<0.001 , the difference is extremely significant (2)Co-culture of GM-CSF and MBP, IL-4 could stimulate DC precursor to express extremely more CD11c. The mean fluorescence intensity of CDllc of study group was856.93 12.45, while the control group was 708 . 5 8 2 1.25 , r = 21.42, P<0.001, the difference is extremely significant. (3) Co-culture of GM-CSF and MBP, IL-3 could up-regulate the expression of CDS a of DC. The mean fluorescence intensity of CD8 a of study group was7751.70 296.63, while the control group was 7279.17 176.77, t = 5.13, P<0...
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