The Expression Of Type Ⅰ Collagen In The Interface Between The Ti6Al4V Alloy Disposed With Surface Oxidation Film And The Bone: An In Vivo Study

ObjectsThe direct orderly connection of structure and function between the implants and the bone, which is called osseointegration , is an important criterion to evaluate the success of the dental implant. Titanium and titanium alloy most widely applies for dental implant materials presently. It is considered that the favorable biocompatibility of titanium and titanium alloy is related to their surface oxidation film. Because the natural oxidation film is too thin, titanium and titanium alloy have been disposed by advanced surface modification technologies to acquire mechanism oxidation film now. In present study the special Ti6A14V alloy disposed with surface oxidation film were implanted in the rats'mandible . Then a histological and immunohistochemical study were put up to investigate the possible role of this special alloy in clinical application.Materials and Methods90 Wister rats were selected into 3 groups randomly, the number of female and male was identical. The three materials, which were Ti6A14V alloy disposed with surface oxidation membran, Ti6A14V alloy and 316L stainless steel alloy ,were made into a cylinder, the diameter 3mm , thickness 1.0 mm. We implanted the three materials in each group. The tissue samples were collected respectively after 1,2, 3,4 and 6 weeks. The samples were analyzed by HE stain , immuno-histochemical stain and Metamorph imaging analyzing system.ResultsExpressions of type I collegan in the interface between the material and the bone in group I (implanted Ti6A14V alloy disposed with surface oxidation film) are better than other groups during each phase (P<0.05). Expressions of type I collegan pairwise compared in each group in each phase are different(P <0.05). They had increased from the first week, reached the peak in the third week, and then declined after the forth week. Earlier formation of synostosis can be seen in HE stained sections.ConclusionsTi6A14V alloy disposed with surface oxidation film is better than other alloys. 316L stainless steel alloy should not be used as dental implant material. Type I collegan can be used as a marker of earlier synostosis formation.