| PrefaceMyometrium contractility is not understood by human being and there have no exact explanation. Recent findings revealed that human placenta and gestational related tissues ( amnion, chorion, decidua ) express human urocortin messenger RNA ( mRNA) gene and localize immunoreactive urocortin. But no information is available on the possible local biological actions. The aim of the present study was to investigate the possible effect of urocortin on myometrial contractility at term,as well as PGF2a and oxytocin in order to explain to relation between urocortin and deliver)'.Materials1 . Human Urocortin ; purchased from American Sigma company.2. Human PGF2a: purchased from American Sigma company.3. Human Oxytocin: purchased from Shanghai Hefeng company.4. Tyrode' s salts: purchased from American Sigma company.5. Meth tube-.China Medical university Energy center.6. YSD -4G poly equipment: AnHui BengBu Medical college factory.7. TB -611 Isometric transducer; China Medical university Energy center.8. RM -6000 recorder; Japan light and electricity company.9. Human myometrial strip: obtained from the upper edge of the uterine incision from 36 elective lower - segment cesarean sections after pregnancy woman agreed.Methods1. Subject ; Myometrail strips were obtained from the upper edge of the uterine incision from 36 elective lower - segment cesarean sections performed before the onset of labour. The specimen was dissected longitudinally into two similar strips which were each suspended vertically in a 30ml meth tube. One end being tied by thin cotton thread to a bottom and the other to a TB -611 isometric transducer. Myometrail contractility was recorded by RM - 6000 recorder.2. Methods;2.1 Direct inotropic effect of Human urocortin: Its direct effect on myometrial strips was tested by adding to one Meth tube of the organ both increasing concentrations of urocortin ( form50 - 500nmol/l , internal 50pg/ml). then recorded Myometrail contractility by RM -6000 recorder. After each single dose tested the strip was repeatedly washes with Tyrode's solution. After test , the viability of the tissue was testing electric stimulation. Specimens that did not respond to e-lectric stimulation were discarded.2. 2 Modulation of PGF2et and PGF2a activity: The two myometrial strips from the same specimen used for each experiment were stimulated with PGF2a( 1.2umol/L) , and recorded myometrail contractility . Then washed by Tyrode' s solution. In test strips, after added urocortin 200 pg/ml ( corresponding meternal plasma concentrations) about 40 minute, throw into PGF2a( 1.2umol/L) in both test strips and control strips . Then recorded myomatrail contractility.Modulation of oxytocin and oxytocin activity : The two myometrial strips from the same specimen used for each experiment were stimulated with oxytocin ( 5 nmol/L) , and recorded myometrail contractility . Then washed by Tyrode's solution. In test strips , after added urocortin 200 pg/ml ( corresponding metemal plasma concentrations ) about 40 minute, throw into oxytocin (5 nmol/L) in both test strips and control strips. Then recorded myomatrail contractility.ResultsUrocortin did not affect myometrial tension development in any of the experimental protocol. The average contraction of control group (0. 6413 0. 279cm) is lower than that of test group (0. 6255 0. 273cm) and the difference between control group and test group was not significant ( P > 0. 05 ) .When PGF2 was added , urocortin increase the myometrial contractility obviously. The average contraction of control group (2. 905 0. 487cm) is lower than that of test group (5. 3038 0. 8751cm) and the difference between control group and test group was significant (PWhen oxytocin was added , urocortin do not increase the myometrial contractility. The average contraction of control group(02.4 0. 33cm )is lower than that of test group (2. 745 0. 3919cm) and the difference between control group and test group was not significant (P >0.05).Discu...
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