| IntroductionGastric carcinoma is one of the most common malignant tumors in peptic tract. Its mortality rate is on the first place among the malignant tumors in many areas in China. Invasion and metastasis is one of the most important biological features of gastric carcinoma and the key factor for survival of patients.The prognosis of gastric carcinoma patients has been improved by the standandization of surgical techniques and the application of multi-modal adjuvant therapy, but there are no effective molecular markers for biological behavior diagnosis. Up to now, much research has been completed to find some distinguishing molecular markers and establish the standard of molecular classification, which may be useful for surgical therapy of gastric carcinoma. Using immunohistochemistry S - P method and RT - PCR technique, the expression of heparanase was tested in gastric carcinoma and para - carcinoma, then the interaction relationship between the expression and clinical - pathological features in gastric carcinoma was investigated to apply object and useful guideline in early diagnosis for the biological behavior, therapy and prognosis in gastric carcinoma.Materials and methodsImmunohistochemistry S - P method and RT - PCR technique were used to test the expression of heparanase in gastric carcinoma and para - carcinoma. All tissue samples were collected from the first clinical academy of China Medical University. 142 cases of primary gastric carcinoma and 20 para - carcinoma were selected, including 10 early gastric carcinoma and 132 advanced ones. For immunostainy, streptavidin peroxidase method was used, the first antibody was heparanase, which was produced in DIAZYME Co. in American. The S - P kit was purchased from Fuzhou Maixin Biocompany. The sections were dewaxed, water processed and then treated by 3% hydrogen peroxide in methanol for 10 minutes to quench the endogenous peroxidase activity. The sections were incubated for 30 minutes with normal non - immuneserum to eliminate nonspecific staining. They were incubated at 4^ with primary antibody. This was followed by incubation with biotin - labelled condary antibody for 30 minutes and streptavition - peroxidase coplx for 30 minutes. The color was developed with diaminobenzidine and the sections were counterstained with harmatoxylin. The blank control were carried out by replacing the primary with PBS. Hie extent of staining was scored as ( - ) indicating <10% of tumor area stained;( +) 10% -20% stained;( ++)20% -50% stained; ( +++ ) >50%. Hie statistical methods were according to test x2 test and correlation analysis.Eight patients were selected who had undergone gastrectomy with node dissction for gastric carcinoma. The neoplastic tissue were collected and separately subjected to RT - PCR analysis with primersspecific for heparanase.Results1. The expression of heparanase by immunohistochemistryHie expression of heparanase was localized in cytoplasm, and brownish yellow granule could be observed . There were no expression in para - carcinoma tissue. The positive rate of early gastric carcinoma was 20% , the advanced was 78.0% , and they were significant different in statistics ( p < 0. 05 ). The positive rate of those with lymph node metastasis(80.5% ) was higher than that without(30.3%). The positive rate of heparanase in well, middle, poorly differentiated ade-nocarcinoma, mucoid adencarcinoma, signet - ring cell carcinoma was 47.1% , 52.2% , 47.4% , 55.6% ,66.3% respectively, and no significant differences were observed among them. It increased in advanced gastric carcinoma as the developing of the depth of wall infiltration ( the superficial is 48.1% , the deep 72.7% ,and the peritoneal 94.1%).2. RT-PCRExpression was observed 4 of 5 cases of gastric carcinoma with lymph node metastasis, and not in those without.Discussion1. The relationship between the expression of tumor.The recent researches suggest that heparanase was a kind of en-doglucoronidase, which was proved to be the proteolyticenzyme of HSPG...
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