| LDL was divided into two subclasses by density gradient ultracen-trifugation, gradient gel electrophoresis: one was big buoyant LDL ( A pattern ) ; the other was small dense LDL ( B pattern). Because of be-ing oxidized easily, pattern B of LDL with a preponderance of small dense LDL particle was regarded as one of the strong dangerous factors in atherosclerotic cerebral infarction (ACI) by most scholars at home and abroad. Atherosclerosis was etiologic basis of ACI. It has not been reported the association of LDL subclass with incidence of ACI yet. The purpose of this experiment was to investigate the association of B pattern LDL subclass with incidence of ACI and to analyze the distribution of diameters and graphs.Experimental Data and method1. SubjectsAccording to the diagnostic criteria from the 4th Chinese cere-bralvescular disease association, 32 ACI hospitalized patients con-firmed by brain GI\MRI were chosen, including 23 males and 9 fe-males , mean age 60.09 ?2. 63years old . All patients were examined in order to exclude some diseases which could affected the plasma lip-id metabolism.Another 32 healthy persons who didn' t suffered any disease were chosen to the control group, including 21 males and 11 females, mean age 49.50 ?. 16 years old.2. Experimental method(1). Collection of blood sample8ml fasting cubital venous blood sample were obtained from the clients in the morning before treatment. 5ml were drawned into tube containing EDTA -Na2(lg/L)for separate LDL,3ml into normal tube for triglyceride ( TG ) , total cholesterol ( TC ) , low - density cholesterol ( LDL - C ) , high density cholesterol ( HDL - C ) , apolipoproteinB ( apo B). Centrifugation immediately (3000 rpm/min, 10 minutes) and then collected the plasma ,. ?70t preserved.(2 ). Separating LDLUsing density gradient ultracentrifugation method. Put 0. 3556g KBr into 0. 9ml plasma to adjust density to 1. 400Kg/L and then put 1. l00Kg/L KBr 1. 8ml into it and put 1. 006Kg/L natrii chloride 9. 3 ml in it. After precise balance, 65000rpm ultracentrifugation 5. 5h, up time 8 min. down time 8 min. Then there was LDL band in the middle of the tube, separated it to 2ml, marked and preserved. Put separated LDL into 3L 0. 001mmol/L PBC, which contain 0.16mmol/ L NaCl 10umol/EDTA,4?C dialyzed 24h and then concentrated it. sealed from air.( 3) Polyacylamide gel electrophoresisThe plasma was electrophoresised by 2. 5% -16% nondenatur-ing polyacylamide gredient gel. Stain 24h and discolored 5 - 6h, scan-ning and measured the areas.(4) LDL areas graphsAccording to LDL particle diameter peaks, scanning every LDL particle areas graphs and measured it. Pattern A is defined as a LDL subclass pattern with the major gradient gel peak at a particle diameter of 25.5nm or greater and presence of a secondary peak of smaller LDL particles. Pattern B has the major peak at a particle diameter of less than 25. 5nm, with skewing of the curve toward larger particle diame-ter. '( 5 ) Examination methodThe level of TG, TC, HDL - C, apoB and LDL - C were meas-ured by oxidatic method.( 6 ) Statistical AnalysisAll data were expressed as mean ?SD. The statistical method was T 梩est, x analysis, correlation analysis.Results1. The comparison of the levels of blood lipid in ACI group with that in the controls.The levels of plasma TG TC LDL - C and apoB in ACI group in-creased more higher than that in controls. ( P <0. 01)2. LDL particles diameter and distributionLDL particle mean diameter was smaller among ACI group than controls( mean ?SD) (24. 85 ?0. 66nm vs. 25. 98 ?0. 63nm ; P < 0.01). The distribution of LDL subclass pattern was significantly different between two groups. ( P < 0. 01)There were 2 A pattern LDL cases, account for 12. 5% ; 14 B pattern LDL cases, account for 87. 5% in ACI group. There were 11 A pattern LDL cases, account for 78. 5% ; 3 B pattern LDL cases, ac-count for 21.43% in the...
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