| Objective: To investigate the effect of Platelet glycoprotein II b/ III a receptor blockade-abciximab on hyperplasia, migration of smooth muscle cells and secretion of platelet-derived growth factor.To envaluate the significance of abciximab in preventing restenosis. Method:Twenty-four New Zealand white rabbit were divided randomly into control group with saline and experimental group with abciximab (iv. 0.25mg/kg). Right carotid arteries in both group were injured with balloon dilation. Then isovolume abciximab and saline solutions were infusedrespectively. Four rabbits were respectively killed in one week, two weeks and four weeks after dilation in each group.Blood sample were drawn predilation and 7 days, 14 days and 28 days of post-dilation in both group. PDGF was checked by means of ELISA.The injuried artery were made paraffin section and hematoxylin eosin stain .KI-67 (proliferation cellular neuclear antigen) and intima area were analyzed by Microscopy and computer graphic . Results: (1) Thrombosis,intima hyperplasia with irregular cellularallay and SMC migration into intima were showed in control group. While ,no thrombosis, no SMC migration and slighty intima hyperplasia were seen in abciximab group. Intimal hyperplasia and SMC migration in abciximab group were not significant compare with control group in two and four weeks of post-dilation. (2)PDGF-BB level was significantly lower in abciximab group than that in control group after one week,two weeks and four weeks of dilation(P<0.05). PDGF-BB level increased significantly after four weeks than that after one and two weeks in abciximab group (P<0.05).In abciximab group,the PDGF-BB level was not significant after one week compared with that after two weeks (P > 0.05) .PDGF-BB level in control group increased by the time and had significance among one, two and four weeks after dilation (P<0.05). (3)KI-67 index was significantly smaller in abciximab group than that in control group after one, two and four weeks (P<0.01). KI-67 index were no different between one week and two weeks in abciximab group (P>0.05). While it was significantly higher after four weeks than that after one and two weeks in abciximab group (P<0. 05).KI-67 index in control group was higher after two weeks than that after one week(P<0.05).KI-67 index after four weeks was higher than that after one and two weeks in control group(p<0.05).(4)Intima area in abciximab group was significantly smaller than that in control group in all observed points(P<0.05).Intima areain abciximab group had no difference after one week and two weeks(P>0.05).Intima area after four weeks was significantly larger than that in one and two weeks after dilation in abciximab (P<0.05).Intima area was significantly different from each other in abciximab and control group (P<0.05). Conclusion: (1) Intima hyperplasia and SMC migration were observed in the vascular dilation injuried model and were inhibited by abciximab.(2)Adhesion and aggregation of platelet led to secretion of active factor, eg.PDGF; Abciximab suppressed the activity of platelet and secretion of PDGF.Abciximab prevent restenosis through poly mechnism.
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