PTA Postoperative Qi And Promoting Blood Detoxification Prescription Drugs Serum Of Rabbit VSMC In RS

The study of pathophysiological mechanism of RS after PTCA indicates inflammatoryreaction and smooth muscle cell proliferation play important roles in development of RS afterPTCA. It's considered in Chinese medicine that the pathogenesis is deficiency in fundamentalaspect and excess in accidental aspect, the fundamental aspect is deficiency in Zhang Qi, theaccidental aspect is blood stasis and sputum turbid. The current preventing and treatingmethods in Chinese medicine are combination of activating blood and removing stasis,removing sputum and dispelling turbid, replenishing Qi and nourishing blood, regulating andnourishing spleen and kidney, treating deficiency and regulating liver. We consider that thepathological process of RS after PTCA is similar to repair in trauma, so the differentiation andtreatment are similar to the treatment of repair in trauma by Chinese medicine. So, theprinciple in Chinese medicine is repairing the new wound, helping the real Qi for Qideficiency, activating the blood and detoxicating according to the mechanism character oflocal combination of stasis and heat, so the heart channel flows fluently, the stasis and hearare removed. The composing of replenishing Qi, activating blood, and detoxicating formula(RADF) embodies this principle.PURPOSE To compare the effect and pharmacological effect of the serum medicine of replenishingQi, activating blood, and detoxicating formula(RADF) which treats trauma and Xuefuzhuyucapsule which is classical formula of activating blood and regulating Qi which can preventand treat RS after PTCA on cultured rabbit VSMC.METHODS 1.Preperation of the serum medicine: ①Replication of animal model and administration:one side common carotid of the rabbits were stimulated by the electricity, meanwhile, theywere fed with high fatty feedstuff, which resulted in local atherosclerosis plaque andthrombosis. The RS model was replicated by balloon in situ expanding and feeding high fattyfeedstuff and PTA, the common carotids of the model were assessed by B-mode ultrasonicsand part pathological section. The feedstuff with replenishing Qi and activating blood formula,Xuefuzhuyu capsule, and modern medicine(Pravastatin, Aspirin) had been given to theanimals for four weeks when the model were replicated; ②Taking of medicine serum: theserum of the model animals and the administrated animals was taken, the serum of the controlgroup animals which received no intervene factor was taken at same time. 2.Primary culture of Rabbit's vascular smooth muscle cell: rabbit's VSMC were primarycultured through tissue anchoring method, the cell form were observed under the invert英文摘要 -5-microscope, and the vascular smooth actin(α-SM actin) was assayed by immunohistoche-mistry method. 3.Observation of effects of serum containing medicine on localizing target cell—VSMCat 12h, 24h: ①Effects of serum containing medicine on VSMC proliferation and cell cycle:the survival rate of VSMC was determined through MTT, the cell cycle, DNA synthesis, andapoptosis were assayed by PI stain and flow cytometer methods;②Effects of serumcontaining medicine on MDA, SOD, NO, NOS concentration: MDA and SOD concentrationwere assayed by MDA test kit trace method and xanthine oxidase method respectively; NOand NOS concentration in the upper liquid were assayed by nitric reduction and NOS test kit;③Effects of serum containing medicine on IL-6, TNF-α, MIP-1, MCP-1 concentration: IL-6,TNF-α, MIP-1, MCP-1 concentration had been tested through double antibody sandwichABC-ELISA method;④Effects of serum containing medicine on hydroxyproline excretion ofVSMC: hydroxyproline concentration in upper liquid of the cultured cell was determinedthrough hydroxyproline chromatometry.RESULTS 1.The serum containing RADF could regulate cell cycle ,and then inhibit VSMCproliferation significantly through regulating G2-M turning point at 12h,G1-S turning point at24h, the MTT...